{"id":45505,"date":"2026-07-04T09:02:39","date_gmt":"2026-07-04T00:02:39","guid":{"rendered":"https:\/\/blog.agentsoft.co.kr\/index.php\/2026\/07\/04\/45505\/"},"modified":"2026-07-04T09:02:39","modified_gmt":"2026-07-04T00:02:39","slug":"mtt-assay-a-report","status":"publish","type":"post","link":"https:\/\/blog.agentsoft.co.kr\/index.php\/2026\/07\/04\/45505\/","title":{"rendered":"MTT assay A+ report"},"content":{"rendered":"

<\/p>\n

\ubaa9\ucc28<\/strong><\/p>\n

1. Introduction
\n2. Materials & Methods
\n3. Result
\n4. Discussion
\n5. References<\/p>\n

\ubcf8\ubb38\ub0b4\uc6a9<\/strong><\/p>\n

-Objective
\n \uf09e Hematocytometer\ub97c \uc774\uc6a9\ud558\uc5ec \uc138\ud3ec \uc218\ub97c \uce21\uc815\ud558\uace0 \uacc4\uc0b0\ud55c\ub2e4.
\n \uf09e MTT assay \uc2e4\ud5d8 \uc6d0\ub9ac\ub97c \ubc14\ud0d5\uc73c\ub85c \uc138\ud3ec \uc0dd\uc874 \ubc0f \uc99d\uc2dd\uc744 \uce21\uc815\ud558\ub294 \ubc29\ubc95\uc744 \uc774\ud574\ud55c\ub2e4.<\/p>\n

-Principle \ubc0f Theory
\n \uf09e Cell counting \uc2dc \uc0ac\uc6a9\ub418\ub294 Trypan blue\uc758 \uc785\uc790\ub294 \uc138\ud3ec\ub9c9\uc744 \ud655\uc0b0\uc758 \ubc29\ubc95\uc73c\ub85c \ud1b5\uacfc\ud558\uc5ec \uc138\ud3ec \ub0b4\ubd80\ub85c \uc720\uc785\ub41c\ub2e4. \uc0b4\uc544\uc788\ub294 \uc138\ud3ec\ub294 ATP \uc5d0\ub108\uc9c0\ub97c \uc774\uc6a9\ud55c exocytosis\ub85c trypan blue \uc785\uc790\ub97c \ub2e4\uc2dc \uc138\ud3ec \uc678\ubd80\ub85c \ubc29\ucd9c\ud558\uc9c0\ub9cc, \uc5d0\ub108\uc9c0\ub97c \uc0dd\uc0b0\ud560 \uc218 \uc5c6\ub294 \uc8fd\uc740 \uc138\ud3ec\ub294 exocytosis\ub97c \ud560 \uc218 \uc5c6\uc5b4 \uccad\uc0c9\uc73c\ub85c \uc5fc\uc0c9\ub41c\ub2e4.
\n\uf09e MTT(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide)\ub294 \uc0b4\uc544\uc788\ub294 \uc138\ud3ec\uc758 \ubbf8\ud1a0\ucf58\ub4dc\ub9ac\uc544 \ub0b4\ub9c9\uc744 \ud1b5\uacfc\ud558\uc5ec \ub300\uc0ac\uc801\uc73c\ub85c \ud65c\uc131\uc744 \uac16\ub294 \uc138\ud3ec\uc5d0 \uc758\ud574 Formazan \uacb0\uc815\uc73c\ub85c \ud658\uc6d0\ub418\uace0, \uc774\ub294 \uc790\uc8fc\uc0c9\uc744 \ub744\ub294 \ube44\uc218\uc6a9\uc131\uc758 \ubd84\uc790\uc774\ub2e4. \ub530\ub77c\uc11c DMSO(Dimethyl sulfoxide)\uc640 \uac19\uc740 \uc720\uae30\uc6a9\ub9e4\uc5d0 \uc758\ud574 \uc6a9\ud574\ub41c Formazan\uc758 \ud761\uad11\ub3c4\ub97c \ud1b5\ud574 \uc138\ud3ec\uc758 \uc99d\uc2dd\uacfc \uc0dd\uc874\uc728 \ubc0f \ubbf8\ud1a0\ucf58\ub4dc\ub9ac\uc544\uc758 \ub300\uc0ac\ud65c\uc131\uc744 \uce21\uc815\ud55c\ub2e4.
\n \uac00. MTT assay\uc758 \uc6d0\ub9ac\uc640 \uc7a5\ub2e8\uc810, \uc751\uc6a9 \uac00\ub2a5\ud55c \ubd84\uc57c\uc5d0 \ub300\ud558\uc5ec \uc870\uc0ac\ud558\uc2dc\uc624.
\n MTT assay\ub294 \uc138\ud3ec\ub300\uc0ac \ud65c\uc131\uc744 \uc0c9 \ubcc0\ud654\ub85c \uce21\uc815\ud558\ub294 \uc2e4\ud5d8\uc73c\ub85c \uc138\ud3ec \ub3c5\uc131 \uc2e4\ud5d8\uc774\ub77c\uace0\ub3c4 \ud55c\ub2e4. Tetrazolium salt\uc778 MTT\ub97c \uc0b4\uc544 \uc788\ub294 \uc138\ud3ec\uc5d0 \ucc98\ub9ac\ud558\uba74, \ubbf8\ud1a0\ucf58\ub4dc\ub9ac\uc544 \uc548\uc758 oxidoreductase enzyme(NADPH)\uc5d0 \uc758\ud574 \ud658\uc6d0\ub418\uc5b4 \ubcf4\ub77c\uc0c9\uc744 \ub760\ub294 formazan \uacb0\uc815\uccb4\ub97c \ud615\uc131\ud55c\ub2e4. \uc8fd\uc740 \uc138\ud3ec\uc758 \uacbd\uc6b0, \ubbf8\ud1a0\ucf58\ub4dc\ub9ac\uc544\uac00 \ud130\uc838 \uc5c6\uc73c\ubbc0\ub85c formazan\uc774 \ud615\uc131\ub418\uc9c0 \uc54a\uace0, \uc0c9\uc744 \ub760\uc9c0 \uc54a\ub294\ub2e4. \uc774\ub807\uac8c \uc0dd\uc131\ub41c formazan\uc740 \ube44\uc218\uc6a9\uc131\uc73c\ub85c, \uc720\uae30\uc6a9\ub9e4 \ub610\ub294 \uacc4\uba74\ud65c\uc131\uc81c \ub4f1\uc5d0 \ub179\uc5ec 570nm\uc5d0\uc11c \ud761\uad11\ub3c4(\uc138\ud3ec\uc758 \ud0c8\uc218\uc18c\ud6a8\uc18c \ud658\uc6d0\ub2a5\ub825)\ub97c \uce21\uc815\ud55c\ub2e4. \uc774 \ub54c, \ud761\uad11\ub3c4\ub294 \uc0b4\uc544 \uc788\ub294 \uc138\ud3ec\uc5d0 \ube44\ub840\ud558\uc5ec \uc99d\uac00\ud558\uac8c \ub41c\ub2e4.<\/p>\n

MTT assay\uc758 \uc7a5\uc810\uc740 \uc138\ud3ec\uac00 \ubd80\ucc29\ub41c \uc0c1\ud0dc\uc5d0\uc11c \uc0dd\uc874\ub3c4\ub97c \ube44\uad50\uc801 \ube60\ub974\uace0 \uc27d\uac8c \uce21\uc815\ud560 \uc218 \uc788\uc73c\uba70, \uc18c\ub7c9\uc758 \uc138\ud3ec\ub85c\ub3c4 \uce21\uc815 \uac00\ub2a5\ud558\ub2e4\ub294 \uc810\uc774\ub2e4. \ud558\uc9c0\ub9cc MTT assay\uac00 \ubbf8\ud1a0\ucf58\ub4dc\ub9ac\uc544 \uae30\ub2a5\uc744 \uae30\ubc18\uc73c\ub85c \ud558\uae30 \ub54c\ubb38\uc5d0 \uc190\uc0c1\ub418\uac70\ub098 \uae30\ub2a5\uc774 \uc774\uc0c1\ud55c \ubbf8\ud1a0\ucf58\ub4dc\ub9ac\uc544\ub97c \uac00\uc9c4 \uc138\ud3ec\uc758 \uc0dd\uc874\ub3c4\ub97c \uc815\ud655\ud558\uac8c \ubc18\uc601\ud558\uc9c0 \ubabb\ud560 \uc218 \uc788\ub2e4\ub294 \ub2e8\uc810\uc774 \uc788\ub2e4. \ub610\ud55c, \uc774 \ubc29\ubc95\uc740 \uc138\ud3ec\ubc00\ub3c4, incubation\uc2dc\uac04, pH \ub4f1\uc758 \uc694\uc778\uc5d0 \uc601\ud5a5\uc744 \ubc1b\uc744 \uc218 \uc788\uae30\uc5d0 \uacb0\uacfc\uc758 \uc815\ud655\uc131\uc5d0 \uc601\ud5a5\uc744 \ubbf8\uce60 \uc218 \uc788\ub2e4.<\/p>\n

\ucd9c\ucc98 : \ud574\ud53c\ucea0\ud37c\uc2a4<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

\ubaa9\ucc28 1. Introduction 2. Materials & Methods 3. Result 4. Discussion 5. References \ubcf8\ubb38\ub0b4\uc6a9 -Objective \uf09e Hematocytometer\ub97c \uc774\uc6a9\ud558\uc5ec \uc138\ud3ec \uc218\ub97c \uce21\uc815\ud558\uace0 \uacc4\uc0b0\ud55c\ub2e4. \uf09e MTT assay \uc2e4\ud5d8 \uc6d0\ub9ac\ub97c \ubc14\ud0d5\uc73c\ub85c \uc138\ud3ec \uc0dd\uc874 \ubc0f \uc99d\uc2dd\uc744 \uce21\uc815\ud558\ub294 \ubc29\ubc95\uc744 \uc774\ud574\ud55c\ub2e4. -Principle \ubc0f Theory \uf09e Cell counting \uc2dc \uc0ac\uc6a9\ub418\ub294 Trypan blue\uc758 \uc785\uc790\ub294 \uc138\ud3ec\ub9c9\uc744 \ud655\uc0b0\uc758 \ubc29\ubc95\uc73c\ub85c \ud1b5\uacfc\ud558\uc5ec \uc138\ud3ec \ub0b4\ubd80\ub85c \uc720\uc785\ub41c\ub2e4. \uc0b4\uc544\uc788\ub294 \uc138\ud3ec\ub294 […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[7376,56113,56112,56114,56111,23367,56110],"class_list":["post-45505","post","type-post","status-publish","format-standard","hentry","category-uncategorized","tag-bca-assay","tag-cell-density","tag-cell-viability","tag-formazan","tag-hematocytometer","tag-mtt-assay","tag-56110"],"_links":{"self":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/45505","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/comments?post=45505"}],"version-history":[{"count":0,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/45505\/revisions"}],"wp:attachment":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/media?parent=45505"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/categories?post=45505"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/tags?post=45505"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}