{"id":46664,"date":"2026-07-16T09:01:36","date_gmt":"2026-07-16T00:01:36","guid":{"rendered":"https:\/\/blog.agentsoft.co.kr\/index.php\/2026\/07\/16\/46664\/"},"modified":"2026-07-16T09:01:36","modified_gmt":"2026-07-16T00:01:36","slug":"%ec%84%b8%ed%8f%ac%ec%83%9d%eb%ac%bc%ed%95%99%ec%8b%a4%ed%97%98-%eb%a7%8c%ec%a0%90a-%ec%8b%a4%ed%97%98-%eb%b3%b4%ea%b3%a0%ec%84%9c%eb%a0%88%ed%8f%ac%ed%8a%b8-03-%eb%a7%88%ec%9a%b0%ec%8a%a4","status":"publish","type":"post","link":"https:\/\/blog.agentsoft.co.kr\/index.php\/2026\/07\/16\/46664\/","title":{"rendered":"\uc138\ud3ec\uc0dd\ubb3c\ud559\uc2e4\ud5d8 \ub9cc\uc810(A+) \uc2e4\ud5d8 \ubcf4\uace0\uc11c(\ub808\ud3ec\ud2b8) 03. \ub9c8\uc6b0\uc2a4 \uc885\uc591 \uc138\ud3ec 4T1(\ub3d9\ubb3c\uc138\ud3ec) \ubc30\uc591 \uc2e4\ud5d8"},"content":{"rendered":"

<\/p>\n

\ubaa9\ucc28<\/strong><\/p>\n

\uff11.\uc11c\ub860 <\/p>\n

\uff12.\uc2e4\ud5d8 \ubaa9\uc801<\/p>\n

\uff13.\uc2e4\ud5d8 \ubc29\ubc95
\n\uac00.\uc2e4\ud5d8 \uc7ac\ub8cc(\ub3c4\uad6c)
\n1)\uc0d8\ud50c
\n2)\uc2dc\uc57d
\n3)\uc7a5\ube44
\n\ub098.\uc2e4\ud5d8 \ubc29\ubc95<\/p>\n

\uff14.\uc2e4\ud5d8 \uacb0\uacfc<\/p>\n

\uff15.\ub17c\uc758 (\uace0\ucc30)<\/p>\n

\uff16.\ucc38\uace0\ubb38\ud5cc<\/p>\n

\ubcf8\ubb38\ub0b4\uc6a9<\/strong><\/p>\n

\uff11.\t\uc11c\ub860
\n\ub3d9\ubb3c\uc138\ud3ec\ub97c \ubc30\uc591\ud558\ub294 \uac83\uc740 \ubaa8\ub4e0 \uc138\ud3ec\uc0dd\ubb3c\ud559 \uc2e4\ud5d8\uc5d0 \uc788\uc5b4\uc11c \uac00\uc7a5 \uae30\ucd08\uc801\uc778 \uc2e4\ud5d8 \ubc29\ubc95\uc73c\ub85c, qRT PCR, MTT assay, Cell Cycle Analysis \ub4f1\uc758 In vitro(\uc2dc\ud5d8\uad00 \ub0b4) \uc218\uc900\uc5d0\uc11c \ud544\uc694\ud55c \uc2e4\ud5d8\uacfc \ub3d9\ubb3c\uc5d0 \uc885\uc591\uc744 \uc774\uc2dd\ud558\ub294 \ub4f1\uc758 In vivo (\uc0dd\uccb4 \ub0b4) \uc218\uc900\uc758 \uc5f0\uad6c\uc5d0\uc11c\ub3c4 \uc0ac\uc6a9\ub418\ub294 \ubc29\ubc95\uc774\ub2e4. \uc774\ubc88\uc5d0\ub294 \uc544\uc8fc \uae30\ubcf8\uc801\uc778 \ub3d9\ubb3c \uc138\ud3ec \ubc30\uc591 \ubc29\ubc95\uc778, \ub9c8\uc6b0\uc2a4 \uc885\uc591 \uc138\ud3ec 4T1\ub97c \ud608\uccad \ubc30\uc9c0\uc5d0 \ubc30\uc591\ud558\ub294 \ubc29\ubc95\uc73c\ub85c \uc2e4\ud5d8\uc744 \uc9c4\ud589\ud560 \uacc4\ud68d\uc774\ub2e4.<\/p>\n

\uff12.\t\uc2e4\ud5d8 \ubaa9\uc801
\nIn vitro(\uc2dc\ud5d8\uad00 \ub0b4) \uc218\uc900\uc5d0\uc11c \uc138\ud3ec\ub97c \ubc30\uc591\ud558\ub294 \ubc29\ubc95\uc744 \uc775\ud788\uace0, \uc774\ub97c \ubc14\ud0d5\uc73c\ub85c \uc2e4\ud5d8 \uc138\ud305 \ubc29\ubc95\uc5d0 \ub300\ud574 \ud559\uc2b5\ud55c\ub2e4.<\/p>\n

\uff13.\t\uc2e4\ud5d8 \ubc29\ubc95
\n\uac00.\t\uc2e4\ud5d8 \uc7ac\ub8cc(\ub3c4\uad6c)
\n1)\t\uc0d8\ud50c
\n–\t\ub9c8\uc6b0\uc2a4 \uc885\uc591 \uc138\ud3ec 4T1(Triple Negative Breast Cancer(TNBC))
\n\ub9c8\uc6b0\uc2a4 \uc885\uc591 \uc138\ud3ec 4T1\uc740 \uc9c4\ud575\uc138\ud3ec\uc774\uc790, \uc0c1\ud53c\uc138\ud3ec\uc774\uba70, \uc131\uc7a5 \uc18d\ub3c4\uac00 \ube60\ub978 \uc138\ud3ec\uc774\ub2e4. \ub9c8\uc6b0\uc2a4\uc5d0\uc11c 4T1 \uc138\ud3ec\uc758 \uc885\uc591 \uc131\uc7a5\uacfc \uc804\uc774 \ud655\uc0b0\uc740 \uc778\uac04\uc5d0\uc11c\uc758 \uc720\ubc29\uc554\uacfc \ub9e4\uc6b0 \uc720\uc0ac\ud558\ub2e4.<\/p>\n

\ub9c8\uc6b0\uc2a4 \uc885\uc591 \uc138\ud3ec 4T1<\/p>\n

2)\t\uc2dc\uc57d
\n–\tDPBS
\nPBS\ub294 Phosphate buffered saline\uc758 \uc57d\uc790\ub85c, nacl\uc744 \ucca8\uac00\ud558\uc5ec \ub9cc\ub4e0 \uc778\uc0b0 \uc644\ucda9\uc6a9\uc561\uc774\ub2e4. \uae30\uc874 PBS\uc5d0 \ud3ec\uc720\ub958 \uc138\ud3ec \ubc30\uc591\uc5d0 \uc880 \ub354 \uc720\uc6a9\ud558\ub3c4\ub85d, 2\uac00\uc774\uc628(Ca2+, Mg2+)\uc744 \uc81c\uac70\ud558\uc5ec \ub9cc\ub4e0 \uc6a9\uc561\uc774 DPBS (Dulbecco’s Phosphate Buffered Saline)\uc774\ub2e4. \ud3ec\uc720\ub958 \uc138\ud3ec \ubc30\uc591\uc5d0\uc11c DPBS\ub294 pH\uc640 \uc0bc\ud22c\uc555\uc744 \uc720\uc9c0\ud558\uace0, \uc138\ud3ec\ub300\uc0ac\uc5d0 \ud544\uc218\uc801\uc778 \ubb34\uae30\uc774\uc628\uc744 \uc81c\uacf5\ud558\ub294 \uc5ed\ud560\uc744 \uc218\ud589\ud558\uace0, \ub610\ud55c \uc2e4\ud5d8\uc5d0\uc11c washing buffer \ub4f1\uc758 \uc6a9\ub3c4\ub85c \uc0ac\uc6a9\ub41c\ub2e4.
\n–\t1X trypsin-EDTA
\n\uc138\ud3ec\ub294 \ubd80\ucc29\ub418\uc5b4 \uc99d\uc2dd\ud558\ub294 Adherent \uc138\ud3ec\uc640 \ud608\uc561 \ub4f1\uc5d0 \ub5a0\ub2e4\ub2c8\uba74\uc11c \uc99d\uc2dd\ud558\ub294 Suspension \uc138\ud3ec\ub85c \ub098\ub25c\ub2e4. Adherent \uc138\ud3ec\ub97c \ubc30\uc591\ud558\uae30 \uc704\ud574\uc11c\ub294, \uc138\ud3ec\uc640 \uc138\ud3ec, \uc138\ud3ec\uc640 \ubc30\uc591\uc6a9\uae30\uc640\uc758 \uc811\ucc29\uc744 \ub5a8\uc5b4\ub728\ub824, \ub2e8\uc77c \uc138\ud3ec\ub85c \ub41c \ud604\ud0c1\uc561\uc73c\ub85c \ub9cc\ub4dc\ub294 \uacfc\uc815\uc774 \ud544\uc694\ud558\ub2e4.<\/p>\n

\ucd9c\ucc98 : \ud574\ud53c\ucea0\ud37c\uc2a4<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

\ubaa9\ucc28 \uff11.\uc11c\ub860 \uff12.\uc2e4\ud5d8 \ubaa9\uc801 \uff13.\uc2e4\ud5d8 \ubc29\ubc95 \uac00.\uc2e4\ud5d8 \uc7ac\ub8cc(\ub3c4\uad6c) 1)\uc0d8\ud50c 2)\uc2dc\uc57d 3)\uc7a5\ube44 \ub098.\uc2e4\ud5d8 \ubc29\ubc95 \uff14.\uc2e4\ud5d8 \uacb0\uacfc \uff15.\ub17c\uc758 (\uace0\ucc30) \uff16.\ucc38\uace0\ubb38\ud5cc \ubcf8\ubb38\ub0b4\uc6a9 \uff11. \uc11c\ub860 \ub3d9\ubb3c\uc138\ud3ec\ub97c \ubc30\uc591\ud558\ub294 \uac83\uc740 \ubaa8\ub4e0 \uc138\ud3ec\uc0dd\ubb3c\ud559 \uc2e4\ud5d8\uc5d0 \uc788\uc5b4\uc11c \uac00\uc7a5 \uae30\ucd08\uc801\uc778 \uc2e4\ud5d8 \ubc29\ubc95\uc73c\ub85c, qRT PCR, MTT assay, Cell Cycle Analysis \ub4f1\uc758 In vitro(\uc2dc\ud5d8\uad00 \ub0b4) \uc218\uc900\uc5d0\uc11c \ud544\uc694\ud55c \uc2e4\ud5d8\uacfc \ub3d9\ubb3c\uc5d0 \uc885\uc591\uc744 \uc774\uc2dd\ud558\ub294 \ub4f1\uc758 In vivo (\uc0dd\uccb4 \ub0b4) \uc218\uc900\uc758 […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[57287,57288,57291,57290,57286,3256,57289,57292],"class_list":["post-46664","post","type-post","status-publish","format-standard","hentry","category-uncategorized","tag-1x-trypsin-edta","tag-cell-culture-media","tag-centrifuge-","tag-clean-bench","tag-dpbs","tag-hemocytometer","tag-trypan-blue-solution","tag-57292"],"_links":{"self":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/46664","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/comments?post=46664"}],"version-history":[{"count":0,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/46664\/revisions"}],"wp:attachment":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/media?parent=46664"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/categories?post=46664"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/tags?post=46664"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}