{"id":46665,"date":"2026-07-16T09:01:39","date_gmt":"2026-07-16T00:01:39","guid":{"rendered":"https:\/\/blog.agentsoft.co.kr\/index.php\/2026\/07\/16\/46665\/"},"modified":"2026-07-16T09:01:39","modified_gmt":"2026-07-16T00:01:39","slug":"%ec%84%b8%ed%8f%ac%ec%83%9d%eb%ac%bc%ed%95%99%ec%8b%a4%ed%97%98-%eb%a7%8c%ec%a0%90a-%ec%8b%a4%ed%97%98-%eb%b3%b4%ea%b3%a0%ec%84%9c%eb%a0%88%ed%8f%ac%ed%8a%b8-04-mtt-assay-wst-8-assay","status":"publish","type":"post","link":"https:\/\/blog.agentsoft.co.kr\/index.php\/2026\/07\/16\/46665\/","title":{"rendered":"\uc138\ud3ec\uc0dd\ubb3c\ud559\uc2e4\ud5d8 \ub9cc\uc810(A+) \uc2e4\ud5d8 \ubcf4\uace0\uc11c(\ub808\ud3ec\ud2b8) 04. MTT assay (WST-8 assay)"},"content":{"rendered":"

<\/p>\n

\ubaa9\ucc28<\/strong><\/p>\n

\uff11.\u00a0\u00a0 \uc11c\ub860
\n\u00a0
\n\uff12.\u00a0\u00a0 \uc2e4\ud5d8 \ubaa9\uc801
\n\u00a0
\n\uff13.\u00a0\u00a0 \uc2e4\ud5d8 \ubc29\ubc95
\n\uac00.\u00a0\u00a0\u00a0 \uc2e4\ud5d8 \uc7ac\ub8cc(\ub3c4\uad6c)
\n1)\u00a0\u00a0\u00a0\u00a0\u00a0 \uc0d8\ud50c
\n2)\u00a0\u00a0\u00a0\u00a0\u00a0 \uc2dc\uc57d
\n3)\u00a0\u00a0\u00a0\u00a0\u00a0 \uc7a5\ube44
\n\ub098.\u00a0\u00a0\u00a0 \uc2e4\ud5d8 \ubc29\ubc95
\n\u00a0
\n\uff14.\u00a0\u00a0 \uc2e4\ud5d8 \uacb0\uacfc
\n\u00a0
\n\uff15.\u00a0\u00a0 \ub17c\uc758 (\uace0\ucc30)
\n\u00a0
\n\uff16.\u00a0\u00a0 \ucc38\uace0\ubb38\ud5cc<\/p>\n

\ubcf8\ubb38\ub0b4\uc6a9<\/strong><\/p>\n

\uff11.\t\uc11c\ub860
\nTetrazolium\uc740 dehydrogenase \ub4f1\uc758 \ud6a8\uc18c\uc5d0 \uc758\ud574 \uc27d\uac8c \ud658\uc6d0\ub418\uc5b4 \uc720\uc0c9\uc758 formazan\uc744 \ud615\uc131\ud55c\ub2e4. \uc774\ub7ec\ud55c \uc6d0\ub9ac\ub85c \uac00\uc7a5 \ub110\ub9ac \uc0ac\uc6a9\ub418\ub294 MTT tetrazolium\uc740 \ub178\ub780\uc0c9 \uc218\uc6a9\uc131 \uae30\uc9c8\ub85c, \ubbf8\ud1a0\ucf58\ub4dc\ub9ac\uc544\uc5d0 \uc788\ub294 reductase\uc5d0 \uc758\ud574 \ud658\uc6d0\ub418\uc5b4 \uccad\uc790\uc0c9\uc744 \ub744\ub294 \ube44\uc218\uc6a9\uc131\uc758 MTT formazan \uc774\ub77c\uace0 \ubd88\ub9ac\ub294 crystal\uc744 \ud615\uc131\ud55c\ub2e4. \ub530\ub77c\uc11c \uc138\ud3ec\uc0ac\uba78\uc5d0 \uc758\ud574 \ubbf8\ud1a0\ucf58\ub4dc\ub9ac\uc544\uac00 \uc81c \uae30\ub2a5\uc744 \ud558\uc9c0 \ubabb\ud558\uac8c \ub418\uba74 MTT\ub97c \ucc98\ub9ac\ud574\ub3c4 formazan\uc744 \ud615\uc131\ud558\uc9c0 \ubabb\ud558\uac8c \ub41c\ub2e4. \uc774\ub7ec\ud55c \ud2b9\uc9d5\uc744 \ud65c\uc6a9\ud558\uc5ec \uc138\ud3ec \uc131\uc7a5 \ubc0f \ub3c5\uc131\uc744 \uce21\uc815\ud558\ub294 MTT assay\uc774 \uc874\uc7ac\ud55c\ub2e4. MTT assay\ub294 \uc57d\ub9ac\ud559, \ub3c5\uc131\ud559 \ub4f1\uc758 \ub2e4\uc591\ud55c \uc2e4\ud5d8\uc2e4\uc5d0\uc11c \ud2b9\uc815 \ubb3c\uc9c8\uc774, \ud2b9\uc815\ud55c \uc0dd\ubb3c\ud559\uc801, \uc0dd\ud654\ud559\uc801 \uae30\ub2a5\uc744 50% \uc5b5\uc81c\ud558\ub294\ub370 \ud544\uc694\ud55c \uc591\uc744 \ub098\ud0c0\ub0b4\ub294 IC50\uc744 \uce21\uc815\ud558\uae30 \uc704\ud55c \uc6a9\ub3c4\ub85c \ub110\ub9ac \uc0ac\uc6a9\ub418\uace0 \uc788\ub2e4.<\/p>\n

\uff12.\t\uc2e4\ud5d8 \ubaa9\uc801
\nMTT tetrazolium\uc744 \uc774\uc6a9\ud558\uc5ec \uc138\ud3ec \uc0ac\uba78\uc5d0 \ub530\ub978 \ubbf8\ud1a0\ucf58\ub4dc\ub9ac\uc544\uc758 \ud65c\uc131\ub3c4 \ucc28\uc774\ub97c \uad00\ucc30\ud55c\ub2e4.<\/p>\n

\uff13.\t\uc2e4\ud5d8 \ubc29\ubc95
\n\uac00.\t\uc2e4\ud5d8 \uc7ac\ub8cc(\ub3c4\uad6c)
\n( * \ud45c\uc2dc\ub294 \uc774\uc804\uc5d0 \uc791\uc131\ud55c \uc2e4\ud5d8 \ubcf4\uace0\uc11c\ub97c \ucc38\uace0\ud55c\ub2e4.)
\n\u2460\t\uc0d8\ud50c
\n–\t\ub9c8\uc6b0\uc2a4 \uc885\uc591 \uc138\ud3ec 4T1(Triple Negative Breast Cancer(TNBC))*
\n\u2461\t\uc2dc\uc57d
\n–\tcell culture media (10% FBS, 1% P\/S) *
\n–\tCCK-8(#K1013,APExBIO)
\n CCK-8\ub294 \u2018Cell Counting Kit 8\u2019\uc758 \uc57d\uc790\ub85c, CCK-8 \uc18d \ud568\uc720\ub418\uc5b4 \uc788\ub294WST-8\uc744 \uc774\uc6a9\ud558\uc5ec \uc138\ud3ec \uc0dd\uc874\uc728, \uc138\ud3ec \ub3c5\uc131 \ub4f1\uc744 \uce21\uc815\ud558\ub294 \ubb3c\uc9c8\uc774\ub2e4. WST-8\ub294 \ud14c\ud2b8\ub77c\uc878\ub9ac\uc6c0(Tetrazolium) \uacc4\uc5f4\uc758 \uad6c\uc131\ubb3c\uc9c8\ub85c, \uc0b4\uc544\uc788\ub294 \uc138\ud3ec\uc5d0\uc11c \ubbf8\ud1a0\ucf58\ub4dc\ub9ac\uc544 \ud0c8\uc218\uc18c\ud6a8\uc18c(dehydrogenase)\uc5d0 \uc758\ud574 \ud658\uc6d0\ub418\uc5b4\uc11c \uc624\ub80c\uc9c0 \uacc4\uc5f4\uc758 \ud3ec\ub974\ub9c8\uc794(formazan)\uc744 \ud615\uc131\ud55c\ub2e4. \uc774\ub54c, \uc0dd\uc131\ub41c \ud3ec\ub974\ub9c8\uc794\uc740 \uc218\uc6a9\uc131\uc774\uace0, \uc27d\uac8c \uc6a9\ud574\ub418\ub294 \ud2b9\uc131\uc744 \uc9c0\ub2c8\uace0 \uc788\ub2e4.
\n\uc989, \ubbf8\ud1a0\ucf58\ub4dc\ub9ac\uc544\uc758 \ud65c\uc131 \uce21\uc815\uc744 \ud1b5\ud574\uc11c, \uc2e4\ud5d8\uc5d0 \uc0ac\uc6a9\ud55c \ud654\ud569\ubb3c\uc774 \uc138\ud3ec\uc5d0 \uc5bc\ub9c8\ub098 \ub3c5\uc131\uc744 \ub098\ud0c0\ub0b4\ub294\uc9c0 \ud655\uc778\ud558\ub294 \uac83\uc774\ub2e4.<\/p>\n

\ucd9c\ucc98 : \ud574\ud53c\ucea0\ud37c\uc2a4<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

\ubaa9\ucc28 \uff11.\u00a0\u00a0 \uc11c\ub860 \u00a0 \uff12.\u00a0\u00a0 \uc2e4\ud5d8 \ubaa9\uc801 \u00a0 \uff13.\u00a0\u00a0 \uc2e4\ud5d8 \ubc29\ubc95 \uac00.\u00a0\u00a0\u00a0 \uc2e4\ud5d8 \uc7ac\ub8cc(\ub3c4\uad6c) 1)\u00a0\u00a0\u00a0\u00a0\u00a0 \uc0d8\ud50c 2)\u00a0\u00a0\u00a0\u00a0\u00a0 \uc2dc\uc57d 3)\u00a0\u00a0\u00a0\u00a0\u00a0 \uc7a5\ube44 \ub098.\u00a0\u00a0\u00a0 \uc2e4\ud5d8 \ubc29\ubc95 \u00a0 \uff14.\u00a0\u00a0 \uc2e4\ud5d8 \uacb0\uacfc \u00a0 \uff15.\u00a0\u00a0 \ub17c\uc758 (\uace0\ucc30) \u00a0 \uff16.\u00a0\u00a0 \ucc38\uace0\ubb38\ud5cc \ubcf8\ubb38\ub0b4\uc6a9 \uff11. \uc11c\ub860 Tetrazolium\uc740 dehydrogenase \ub4f1\uc758 \ud6a8\uc18c\uc5d0 \uc758\ud574 \uc27d\uac8c \ud658\uc6d0\ub418\uc5b4 \uc720\uc0c9\uc758 formazan\uc744 \ud615\uc131\ud55c\ub2e4. \uc774\ub7ec\ud55c \uc6d0\ub9ac\ub85c \uac00\uc7a5 \ub110\ub9ac \uc0ac\uc6a9\ub418\ub294 MTT tetrazolium\uc740 \ub178\ub780\uc0c9 […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[23369,57294,12093,56114,57295,23367,23370,57293,37031],"class_list":["post-46665","post","type-post","status-publish","format-standard","hentry","category-uncategorized","tag-cck-8-assay","tag-docetaxel","tag-elisa","tag-formazan","tag-ic50","tag-mtt-assay","tag-tetrazolium","tag-wst-8-assay","tag-37031"],"_links":{"self":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/46665","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/comments?post=46665"}],"version-history":[{"count":0,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/46665\/revisions"}],"wp:attachment":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/media?parent=46665"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/categories?post=46665"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/tags?post=46665"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}