{"id":46695,"date":"2026-07-16T09:02:46","date_gmt":"2026-07-16T00:02:46","guid":{"rendered":"https:\/\/blog.agentsoft.co.kr\/index.php\/2026\/07\/16\/46695\/"},"modified":"2026-07-16T09:02:46","modified_gmt":"2026-07-16T00:02:46","slug":"%ec%84%b8%ed%8f%ac%ec%83%9d%eb%ac%bc%ed%95%99%ec%8b%a4%ed%97%98-%eb%a7%8c%ec%a0%90a-%ec%8b%a4%ed%97%98-%eb%b3%b4%ea%b3%a0%ec%84%9c%eb%a0%88%ed%8f%ac%ed%8a%b8-09-tunel-staining","status":"publish","type":"post","link":"https:\/\/blog.agentsoft.co.kr\/index.php\/2026\/07\/16\/46695\/","title":{"rendered":"\uc138\ud3ec\uc0dd\ubb3c\ud559\uc2e4\ud5d8 \ub9cc\uc810(A+) \uc2e4\ud5d8 \ubcf4\uace0\uc11c(\ub808\ud3ec\ud2b8) 09. TUNEL staining"},"content":{"rendered":"

<\/p>\n

\ubaa9\ucc28<\/strong><\/p>\n

\uff11.\u00a0\u00a0 \uc11c\ub860
\n\u00a0
\n\uff12.\u00a0\u00a0 \uc2e4\ud5d8 \ubaa9\uc801
\n\u00a0
\n\uff13.\u00a0\u00a0 \uc2e4\ud5d8 \ubc29\ubc95
\n\uac00.\u00a0\u00a0\u00a0 \uc2e4\ud5d8 \uc7ac\ub8cc(\ub3c4\uad6c)
\n1)\u00a0\u00a0\u00a0\u00a0\u00a0 \uc0d8\ud50c
\n2)\u00a0\u00a0\u00a0\u00a0\u00a0 \uc2dc\uc57d
\n3)\u00a0\u00a0\u00a0\u00a0\u00a0 \uc7a5\ube44
\n\ub098.\u00a0\u00a0\u00a0 \uc2e4\ud5d8 \ubc29\ubc95
\n\u00a0
\n\uff14.\u00a0\u00a0 \uc2e4\ud5d8 \uacb0\uacfc
\n\u00a0
\n\uff15.\u00a0\u00a0 \ub17c\uc758 (\uace0\ucc30)
\n\u00a0
\n\uff16.\u00a0\u00a0 \ucc38\uace0\ubb38\ud5cc<\/p>\n

\ubcf8\ubb38\ub0b4\uc6a9<\/strong><\/p>\n

\uba74\uc5ed \uc5fc\uc0c9\ubc95(Immunostaining)\uc740 \uc138\ud3ec \ub610\ub294 \uc870\uc9c1\uc5d0\uc11c \ud2b9\uc815 \ubd84\uc790, \ub2e8\ubc31\uc9c8 \ub4f1\uc744 \ud56d\uccb4\ub97c \uc774\uc6a9\ud574 \uac00\uc2dc\ud654\ud558\ub294 \ubc29\ubc95\uc73c\ub85c, \ud2b9\uc815 \ub2e8\ubc31\uc9c8\uc5d0 \uc120\ud0dd\uc801\uc73c\ub85c \uacb0\ud569\ud558\ub294 \ud56d\uccb4\ub97c \uc138\ud3ec\ub098 \uc870\uc9c1\uc5d0 \ubc18\uc751\uc2dc\ud0a8 \ud6c4, \ud56d\uccb4\uc758 \uacb0\ud569 \ubc18\uc751 \ud6c4 \uadf8 \ud56d\uccb4\uc758 \uc704\uce58\ub97c \uad00\ucc30\ud558\ub294 \uc0dd\ud654\ud559\uc801\uc778 \ubc29\ubc95\uc774\ub2e4. \uba74\uc5ed\uc5fc\uc0c9\ubc95\uc758 \uc885\ub958\ub85c\ub294 \uba74\uc5ed \uc870\uc9c1 \ud654\ud559 \uc5fc\uc0c9\ubc95 (immunohistochemistry, IHC), \uba74\uc5ed \uc138\ud3ec \ud654\ud559 \uc5fc\uc0c9\ubc95 (immunocytochemistry, ICC), \uba74\uc5ed \ud615\uad11\ubc95 (immunofluorescence, IF) \ub4f1\uc774 \uc874\uc7ac\ud55c\ub2e4.
\n\uba74\uc5ed \uc870\uc9c1 \ud654\ud559 \uc5fc\uc0c9\ubc95 (IHC)\uc740 \ud2b9\uc815 \ud0c0\uac9f \ub2e8\ubc31\uc9c8\uc774 \uace0\uc815\ub41c \uc870\uc9c1 \ub0b4\uc5d0\uc11c \ubc1c\ud604\ud558\ub294\uc9c0, \ubc1c\ud604\ud55c\ub2e4\uba74 \uc5b4\ub514 \uc704\uce58\uc5d0\uc11c \ubc1c\ud604\ud558\ub294\uc9c0\uc5d0 \ub300\ud574 \uc870\uc9c1\uc5d0 \uc788\ub294 \ud2b9\uc815 \ud56d\uc6d0\uc5d0 \uacb0\ud569\ud558\ub294 \ud2b9\uc815 \ud56d\uccb4\ub97c \uc774\uc6a9\ud558\uc5ec \uc5fc\uc0c9\ud558\ub294 \ubc29\ubc95\uc774\ub2e4. IHC\ub294 \uc774\ucc28\ud56d\uccb4 \uc5fc\uc0c9 \uc774\ud6c4 \ubc1c\uc0c9\uc81c\uc778 DAB \ub4f1 \uc744 \uc774\uc6a9\ud558\uc5ec \ubc1c\uc0c9\ud558\ub294 \ubc29\ubc95\uc73c\ub85c \ubcf4\ud1b5 peroxidase\uc5d0 \uc758\ud574 \uc0b0\ud654\ub418\uc5b4 \uac08\uc0c9\uc73c\ub85c \ubc1c\uc0c9\ub41c\ub2e4. \ucd5c\uadfc\uc5d0\ub294 \uac08\uc0c9\uc73c\ub85c \ubc18\uc751\ud558\ub294 DAB \ubc18\uc751 \ub9d0\uace0\ub3c4 \ub2e4\uc591\ud55c \uc885\ub958\uc758 kit\ub4e4\uc774 \uac1c\ubc1c\ub418\uace0 \uc788\ub2e4.
\n\uba74\uc5ed \uc138\ud3ec \ud654\ud559 \uc5fc\uc0c9\ubc95 (ICC)\uc740 IHC\uc640 \uac70\uc758 \uc720\uc0ac\ud558\uc9c0\ub9cc, IHC\uac00 \uc870\uc9c1\uc744 \uc5fc\uc0c9\ud558\uc5ec \uad00\ucc30\ud55c\ub2e4\ub294 \uc810\uacfc\ub294 \ub2ec\ub9ac, \uc138\ud3ec\ub97c \uc5fc\uc0c9\ud558\uc5ec \uad00\ucc30\ud558\ub294 \uba74\uc5ed\uc5fc\uc0c9\ubc95\uc774\ub2e4.
\n\uba74\uc5ed \ud615\uad11\ubc95 (IF)\uc740 \uc870\uc9c1 \ud56d\uccb4\uc758 \uc77c\ucc28 \ud56d\uccb4\ub97c \uacb0\ud569\uc2dc\ud0a4\uace0, \ud615\uad11 \ud0d0\uce68\uc774 \ubd80\ucc29\ub41c 2\ucc28 \ud56d\uccb4\ub97c 1\ucc28 \ud56d\uccb4\uc640 \uc120\ud0dd\uc801\uc73c\ub85c \uacb0\ud569\uc2dc\ud0a4\ub294 \ubc29\ubc95\uc73c\ub85c, \ud615\uad11 \ud604\ubbf8\uacbd\uc744 \ud1b5\ud574 \uad00\ucc30\ud55c \uc2dc\ub8cc \ub0b4\uc5d0\uc11c \uc5ec\ub7ec \uac1c\uc758 \ud56d\uc6d0\uc744 \uac01\uac01 \uac00\uc2dc\ud654 \ud560 \uc218 \uc788\ub2e4\ub294 \uc7a5\uc810\uc744 \uc9c0\ub2cc \uc2e4\ud5d8\ubc95\uc774\ub2e4. \uba74\uc5ed \ud615\uad11\ubc95\uc744 \ud1b5\ud574\uc11c, \uc870\uc9c1\uc5d0\uc11c\uc758 \ud2b9\uc815 \ub2e8\ubc31\uc9c8\uc758 \ubc1c\ud604\uc704\uce58, \ubc1c\ud604 \uc815\ub3c4\ub97c \ud655\uc778\ud560 \uc218 \uc788\ub2e4. IF\ub294 IHC\uc640 \uac70\uc758 \uc720\uc0ac\ud55c \ud504\ub85c\ud1a0\ucf5c\uc744 \uc9c0\ub2c8\uace0 \uc788\ub294\ub370, \ucc28\uc774\uc810\uc73c\ub85c\ub294 IHC\uc640 \ub2ec\ub9ac \uc774\ucc28 \ud56d\uccb4\ub85c \ud615\uad11\uc774 \ub2ec\ub9b0 \ud56d\uccb4\ub97c \uc0ac\uc6a9\ud558\uba70, H2O2 incubation \uacfc\uc815\uc774 \ud544\uc694\ud558\uc9c0 \uc54a\ub294\ub2e4\ub294 \uc810\uc774 \uc874\uc7ac\ud55c\ub2e4.<\/p>\n

\ucd9c\ucc98 : \ud574\ud53c\ucea0\ud37c\uc2a4<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

\ubaa9\ucc28 \uff11.\u00a0\u00a0 \uc11c\ub860 \u00a0 \uff12.\u00a0\u00a0 \uc2e4\ud5d8 \ubaa9\uc801 \u00a0 \uff13.\u00a0\u00a0 \uc2e4\ud5d8 \ubc29\ubc95 \uac00.\u00a0\u00a0\u00a0 \uc2e4\ud5d8 \uc7ac\ub8cc(\ub3c4\uad6c) 1)\u00a0\u00a0\u00a0\u00a0\u00a0 \uc0d8\ud50c 2)\u00a0\u00a0\u00a0\u00a0\u00a0 \uc2dc\uc57d 3)\u00a0\u00a0\u00a0\u00a0\u00a0 \uc7a5\ube44 \ub098.\u00a0\u00a0\u00a0 \uc2e4\ud5d8 \ubc29\ubc95 \u00a0 \uff14.\u00a0\u00a0 \uc2e4\ud5d8 \uacb0\uacfc \u00a0 \uff15.\u00a0\u00a0 \ub17c\uc758 (\uace0\ucc30) \u00a0 \uff16.\u00a0\u00a0 \ucc38\uace0\ubb38\ud5cc \ubcf8\ubb38\ub0b4\uc6a9 \uba74\uc5ed \uc5fc\uc0c9\ubc95(Immunostaining)\uc740 \uc138\ud3ec \ub610\ub294 \uc870\uc9c1\uc5d0\uc11c \ud2b9\uc815 \ubd84\uc790, \ub2e8\ubc31\uc9c8 \ub4f1\uc744 \ud56d\uccb4\ub97c \uc774\uc6a9\ud574 \uac00\uc2dc\ud654\ud558\ub294 \ubc29\ubc95\uc73c\ub85c, \ud2b9\uc815 \ub2e8\ubc31\uc9c8\uc5d0 \uc120\ud0dd\uc801\uc73c\ub85c \uacb0\ud569\ud558\ub294 \ud56d\uccb4\ub97c \uc138\ud3ec\ub098 \uc870\uc9c1\uc5d0 […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[57261,57368,56400,57369,57367,57363,57365,57362,57364,57366],"class_list":["post-46695","post","type-post","status-publish","format-standard","hentry","category-uncategorized","tag-apoptosis","tag-dutp","tag-immunostaining","tag-pbs-t","tag-tdt-","tag-tunel-staining","tag-----icc","tag-57362","tag-----ihc","tag---if"],"_links":{"self":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/46695","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/comments?post=46695"}],"version-history":[{"count":0,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/46695\/revisions"}],"wp:attachment":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/media?parent=46695"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/categories?post=46695"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/tags?post=46695"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}