{"id":47607,"date":"2026-07-25T09:03:35","date_gmt":"2026-07-25T00:03:35","guid":{"rendered":"https:\/\/blog.agentsoft.co.kr\/index.php\/2026\/07\/25\/47607\/"},"modified":"2026-07-25T09:03:35","modified_gmt":"2026-07-25T00:03:35","slug":"09_pcr%ec%9d%84%ec%9d%b4%ec%9a%a9%ed%95%9cdna%eb%b3%b5%ec%a0%9c%ec%99%80dna%ec%a0%84%ea%b8%b0%ec%98%81%eb%8f%99%eb%b3%b4%ea%b3%a0%ec%84%9c","status":"publish","type":"post","link":"https:\/\/blog.agentsoft.co.kr\/index.php\/2026\/07\/25\/47607\/","title":{"rendered":"09_PCR\uc744\uc774\uc6a9\ud55cDNA\ubcf5\uc81c\uc640DNA\uc804\uae30\uc601\ub3d9\ubcf4\uace0\uc11c"},"content":{"rendered":"<p><img decoding=\"async\" src=\"https:\/\/image4.happycampus.com\/Production\/thumbnail\/2024\/07\/09\/data30111077-0001.jpg\"><img decoding=\"async\" src=\"https:\/\/image4.happycampus.com\/Production\/thumbnail\/2024\/07\/09\/data30111077-0002.jpg\"><\/p>\n<p><strong>\ubaa9\ucc28<\/strong><\/p>\n<p>1.\t\uc2e4\ud5d8 \ubaa9\uc801<br \/>\n2.\t\uc2e4\ud5d8 \uc6d0\ub9ac<br \/>\n3.\t\uc2e4\ud5d8 \uc7ac\ub8cc \ubc0f \ubc29\ubc95<br \/>\n4.\t\uc2e4\ud5d8 \uacb0\uacfc<br \/>\n5.\t\uc2e4\ud5d8\uc5d0 \ub300\ud55c \uace0\ucc30<br \/>\n6.\t\ucc38\uace0 \ubb38\ud5cc<\/p>\n<p><strong>\ubcf8\ubb38\ub0b4\uc6a9<\/strong><\/p>\n<p>1. \uc2e4\ud5d8 \ubaa9\uc801<br \/>\nPCR\uacfc \uc804\uae30\uc601\ub3d9\uc758 \uc6d0\ub9ac\ub97c \uc774\ud574\ud558\uace0, \uc9c1\uc811 \ubcf5\uc81c\ud55c DNA\ub97c \uc804\uae30\uc601\ub3d9\uc744 \ud1b5\ud574 DNA ladder\uc640 \ube44\uad50\ud55c\ub2e4.<\/p>\n<p>2. \uc2e4\ud5d8 \uc6d0\ub9ac<br \/>\n(1) PCR<br \/>\n\u25cf PCR: \uc911\ud569\ud6a8\uc18c \uc5f0\uc1c4\ubc18\uc751. 1983\ub144 K. B. Mullis\uac00 \uace0\uc548\ud55c \ubc29\ubc95\uc73c\ub85c, DNA\ub97c \ub300\ub7c9\uc73c\ub85c \uc99d\ud3ed\ud560 \uc218 \uc788\ub2e4.<br \/>\n\u25cf PCR \uc7ac\ub8cc: \ubcf5\uc81c\ud558\uace0\uc790 \ud558\ub294 DNA, Taq DNA \uc911\ud569\ud6a8\uc18c, dNTPs(dATP, dGTP, dCTP, dTTP), \ud504\ub77c\uc774\uba38, DNA \uc911\ud569\ud6a8\uc18c\uc6a9 \uc644\ucda9\uc6a9\uc561, MgCl2<br \/>\n\uf0a7 \uc8fc\ud615 DNA: \ubcf4\ud1b5 20~100ng\uc758 DNA\ub97c \uc0ac\uc6a9\ud55c\ub2e4. A-T, G-C \ube44\uc728\uc774 \ube44\uc2b7\ud55c \uac83\uc774 \uc88b\ub2e4. G-C\uc758 \ubd84\ud3ec\ub294 \ubab0\ub824 \uc788\uc9c0 \uc54a\uc740 \uac83\uc744 \uc0ac\uc6a9\ud55c\ub2e4.<br \/>\n\uf0a7 Taq DNA \uc911\ud569\ud6a8\uc18c1: \uace0\uc628\uc5d0\uc11c \uc0ac\ub294 thermus aquaticus \ubc15\ud14c\ub9ac\uc544\uc5d0\uc11c \uae30\uc6d0\ud55c DNA \uc911\ud569\ud6a8\uc18c\ub85c 90\u2103 \uc774\uc0c1\uc5d0\uc11c\ub3c4 \ud65c\uc131\uc744 \uc783\uc9c0 \uc54a\ub294\ub2e4. \ud65c\uc131\uc758 \ucd5c\uc801\uc628\ub3c4\ub294 75~80\u2103 \uc815\ub3c4\uc774\ub2e4. KCl\uc774\ub098 Mg2+ \uc774\uc628 \ub4f1\uc774 Taq \uc911\ud569\ud6a8\uc18c\uc758 \ud65c\uc131\uc744 \uc99d\uac00\uc2dc\ud0a8\ub2e4.<br \/>\n\u25cf \ub354\ubb34\uc2a4 \uc544\ucfe0\uc544\ud2f0\ucfe0\uc2a4(thermus aquaticus)2: \uc610\ub85c\uc2a4\ud1a4 \uad6d\ub9bd\uacf5\uc6d0\uc758 \uc628\ucc9c\uc5d0\uc11c \ucd5c\ucd08\ub85c \ubc1c\uacac\ub41c \uc628\ucc9c\uc774\ub098 \ubc14\ub2f7\uc18d \uc5f4\uc218 \uad6c\uba4d\uc5d0 \uc0ac\ub294 \ubc15\ud14c\ub9ac\uc544. \uadf8\ub78c \uc74c\uc131\uade0 \uc911 \ud558\ub098\ub85c \uc77c\ubc18\uc801\uc73c\ub85c \ub9c9\ub300 \ubaa8\uc591\uc73c\ub85c \uc874\uc7ac\ud558\uba70, \uacbd\uc6b0\uc5d0 \ub530\ub77c\uc11c\ub294 \uc2e4 \ubaa8\uc591\uc744 \ub760\uae30\ub3c4 \ud55c\ub2e4.<br \/>\n\uf0a7 DNA \uc911\ud569\ud6a8\uc18c\uc6a9 \uc644\ucda9\uc6a9\uc561: \uc911\ud569\ud6a8\uc18c\uc758 \ud65c\uc131\ud654\ub97c \uc704\ud574 \ud544\uc694\ud558\ub2e4.<br \/>\n\uf0a7 MgCl2: \uc644\ucda9\uc6a9\uc561\uc5d0 \ub4e4\uc5b4\uac04\ub2e4. Taq \uc911\ud569\ud6a8\uc18c\uc758 \ud65c\uc131\uc744 \ub3d5\ub294\ub2e4.<br \/>\n\u25cf PCR \uacfc\uc8153:<br \/>\n\u2460 \ubcc0\uc131 \ub2e8\uacc4: \uc57d 95\u2103\uc5d0\uc11c \uc77c\uc5b4\ub098\ub294 \ub2e8\uacc4. \uc99d\ud3ed\ud560 \uc774\uc911\uac00\ub2e5 DNA\uac00\ub2e5\uc744 \uace0\uc628\uc5d0\uc11c \uc5f4\ubcc0\uc131\ud558\uc5ec \ub2e8\uc77c \uac00\ub2e5\uc73c\ub85c \ubd84\ub9ac\ud55c\ub2e4.<br \/>\n\u2461 \uacb0\ud569 \ub2e8\uacc4: \uc57d 50\u2103\uc5d0\uc11c \uc77c\uc5b4\ub098\ub294 \ub2e8\uacc4. \uc0ac\uc6a9\ud558\ub294 \ud504\ub77c\uc774\uba38\uc5d0 \ub530\ub77c \uc628\ub3c4\uac00 \ub2ec\ub77c\uc9c8 \uc218 \uc788\ub2e4. \ud504\ub77c\uc774\uba38\uac00 \uc790\uc2e0\uacfc \uc0c1\ubcf4\uc801\uc778 \uc5fc\uae30\ub97c \uac00\uc9c0\uace0 \uc788\ub294 \uc8fc\ud615 DNA\uc5d0 \uacb0\ud569\ud558\ub294 \ub2e8\uacc4\uc774\ub2e4. G-C \uc0ac\uc774 \uc218\uc18c\uacb0\ud569\uc774 \ub354 \uac15\ud558\ubbc0\ub85c \uc77c\ubc18\uc801\uc73c\ub85c G-C\uacb0\ud569\uc774 50%\uc774\uc0c1\uc778 \ud504\ub77c\uc774\uba38\ub97c \uc0ac\uc6a9\ud55c\ub2e4.<br \/>\n\u2462 \uc911\ud569 \ub2e8\uacc4: \uc57d 72\u2103\uc5d0\uc11c \uc77c\uc5b4\ub098\ub294 \ub2e8\uacc4. DNA \uc911\ud569\ud6a8\uc18c\uac00 \ud504\ub77c\uc774\uba38\uc5d0 \uc774\uc5b4 5\u2019 \u2192 3\u2019 \ubc29\ud5a5\uc73c\ub85c DNA\ub97c \ud569\uc131\ud55c\ub2e4.<\/p>\n<p>\ucd9c\ucc98 : <a href=\"https:\/\/www.happycampus.com\/report-doc\/30111077\/\" target=\"_blank\">\ud574\ud53c\ucea0\ud37c\uc2a4<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>\ubaa9\ucc28 1. \uc2e4\ud5d8 \ubaa9\uc801 2. \uc2e4\ud5d8 \uc6d0\ub9ac 3. \uc2e4\ud5d8 \uc7ac\ub8cc \ubc0f \ubc29\ubc95 4. \uc2e4\ud5d8 \uacb0\uacfc 5. \uc2e4\ud5d8\uc5d0 \ub300\ud55c \uace0\ucc30 6. \ucc38\uace0 \ubb38\ud5cc \ubcf8\ubb38\ub0b4\uc6a9 1. \uc2e4\ud5d8 \ubaa9\uc801 PCR\uacfc \uc804\uae30\uc601\ub3d9\uc758 \uc6d0\ub9ac\ub97c \uc774\ud574\ud558\uace0, \uc9c1\uc811 \ubcf5\uc81c\ud55c DNA\ub97c \uc804\uae30\uc601\ub3d9\uc744 \ud1b5\ud574 DNA ladder\uc640 \ube44\uad50\ud55c\ub2e4. 2. \uc2e4\ud5d8 \uc6d0\ub9ac (1) PCR \u25cf PCR: \uc911\ud569\ud6a8\uc18c \uc5f0\uc1c4\ubc18\uc751. 1983\ub144 K. B. Mullis\uac00 \uace0\uc548\ud55c \ubc29\ubc95\uc73c\ub85c, DNA\ub97c \ub300\ub7c9\uc73c\ub85c [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[9301,425,10828,2387,58061,3799,7236],"class_list":["post-47607","post","type-post","status-publish","format-standard","hentry","category-uncategorized","tag-__aa","tag-12-","tag-10828","tag-2387","tag-58061","tag-3799","tag-7236"],"_links":{"self":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/47607","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/comments?post=47607"}],"version-history":[{"count":0,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/47607\/revisions"}],"wp:attachment":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/media?parent=47607"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/categories?post=47607"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/tags?post=47607"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}