{"id":47956,"date":"2026-07-29T09:01:18","date_gmt":"2026-07-29T00:01:18","guid":{"rendered":"https:\/\/blog.agentsoft.co.kr\/index.php\/2026\/07\/29\/47956\/"},"modified":"2026-07-29T09:01:18","modified_gmt":"2026-07-29T00:01:18","slug":"%ec%8b%9d%eb%ac%bc%eb%b6%84%ec%9e%90%ec%83%9d%eb%ac%bc%ed%95%99%ec%8b%a4%ed%97%98-agrobacterium-transformation-%ea%b2%b0%ea%b3%bc%eb%b3%b4%ea%b3%a0%ec%84%9c","status":"publish","type":"post","link":"https:\/\/blog.agentsoft.co.kr\/index.php\/2026\/07\/29\/47956\/","title":{"rendered":"\uc2dd\ubb3c\ubd84\uc790\uc0dd\ubb3c\ud559\uc2e4\ud5d8 Agrobacterium Transformation \uacb0\uacfc\ubcf4\uace0\uc11c"},"content":{"rendered":"

<\/p>\n

\ubaa9\ucc28<\/strong><\/p>\n

1.\t\uc2e4\ud5d8\uc81c\ubaa9<\/p>\n

2.\t \uc791\uc131\uc77c\uc790<\/p>\n

3.\t \uc2e4\ud5d8\uac1c\uc694<\/p>\n

4.\t\uc2e4\ud5d8\uc774\ub860
\n\uac00) Agrobacterium competent cell (GV3101)\uc774\ub780
\n\ub098) Freeze-Thaw method\ub780
\n\ub2e4) Binary bector\ub780
\n\ub77c) YEP Media\ub780<\/p>\n

5.\tMaterials and methods
\n\uac00) Materials
\n\ub098) Methods<\/p>\n

6.\t\uc2e4\ud5d8\uacb0\uacfc<\/p>\n

7.\tDiscussion<\/p>\n

\ubcf8\ubb38\ub0b4\uc6a9<\/strong><\/p>\n

\uac00) Agrobacterium competent cell (GV3101)\uc774\ub780
\nGV3101\uc740 Agrobacterium tumefaciens Chemically Competent Cell\uc758 \uc77c\uc885\uc73c\ub85c, \uc560\uae30\uc7a5\ub300, \ub2f4\ubc30, \uc625\uc218\uc218, \uac10\uc790 \ub4f1\uc758 \uc2dd\ubb3c\uc744 \ud615\uc9c8 \uc804\ud658\ud558\ub294\ub370 \uc0ac\uc6a9\ub420 \uc218 \uc788\ub2e4. \uc5ec\uae30\uc5d0\ub294 screening\uc744 \uc704\ud55c rifampicin resistant gene (rif )\uc774 \ubbf8\ub9ac \ud3ec\ud568\ub418\uc5b4 \uc788\uc73c\uba70, gentamicin resistance gene \uacfc vir gene\uc744 \ud3ec\ud568\ud55c Ti plasmid\ub97c \uc6b4\ubc18\ud560 \uc218 \uc788\ub2e4.(1)<\/p>\n

\ub098) Freeze-Thaw method\ub780
\nFreeze-Thaw method\ub294 Dityatkin et al.\uc5d0 \uc758\ud558\uc5ec E.coli\uc5d0 \ub300\ud55c \uc5f0\uad6c\ub97c \ud1b5\ud558\uc5ec 1972\ub144 \ucc98 \uc74c\uc73c\ub85c \uc81c\uc548\ub41c \ubc29\ubc95\uc73c\ub85c \uc774 \uae30\uc220\uc744 \uc0ac\uc6a9\ud558\uba74 DNA \ubd84\uc790\uac00 cell wall\uacfc membrane\uc758 temporary lesions\uc744 \ud1b5\ud574 passive diffusion\uc744 \ud1b5\ud558\uc5ec \ub3d9\uacb0-\ud574\ub3d9\ub41c \uc138\ud3ec\uc5d0 \uce68\ud22c\ud55c\ub2e4.(2)<\/p>\n

\ucd9c\ucc98 : \ud574\ud53c\ucea0\ud37c\uc2a4<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

\ubaa9\ucc28 1. \uc2e4\ud5d8\uc81c\ubaa9 2. \uc791\uc131\uc77c\uc790 3. \uc2e4\ud5d8\uac1c\uc694 4. \uc2e4\ud5d8\uc774\ub860 \uac00) Agrobacterium competent cell (GV3101)\uc774\ub780 \ub098) Freeze-Thaw method\ub780 \ub2e4) Binary bector\ub780 \ub77c) YEP Media\ub780 5. Materials and methods \uac00) Materials \ub098) Methods 6. \uc2e4\ud5d8\uacb0\uacfc 7. Discussion \ubcf8\ubb38\ub0b4\uc6a9 \uac00) Agrobacterium competent cell (GV3101)\uc774\ub780 GV3101\uc740 Agrobacterium tumefaciens Chemically Competent Cell\uc758 \uc77c\uc885\uc73c\ub85c, \uc560\uae30\uc7a5\ub300, \ub2f4\ubc30, \uc625\uc218\uc218, \uac10\uc790 \ub4f1\uc758 \uc2dd\ubb3c\uc744 \ud615\uc9c8 […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[58365,25186,12090,58366,58369,58368,58367,58370,58364],"class_list":["post-47956","post","type-post","status-publish","format-standard","hentry","category-uncategorized","tag-agrobacterium","tag-perovskite","tag-transformation","tag-58366","tag-58369","tag-58368","tag-58367","tag-58370","tag-58364"],"_links":{"self":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/47956","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/comments?post=47956"}],"version-history":[{"count":0,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/47956\/revisions"}],"wp:attachment":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/media?parent=47956"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/categories?post=47956"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/tags?post=47956"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}