{"id":47960,"date":"2026-07-29T09:01:28","date_gmt":"2026-07-29T00:01:28","guid":{"rendered":"https:\/\/blog.agentsoft.co.kr\/index.php\/2026\/07\/29\/47960\/"},"modified":"2026-07-29T09:01:28","modified_gmt":"2026-07-29T00:01:28","slug":"%ec%8b%9d%eb%ac%bc%eb%b6%84%ec%9e%90%ec%83%9d%eb%ac%bc%ed%95%99%ec%8b%a4%ed%97%98-histochemical-gus-assay-%ea%b2%b0%ea%b3%bc%eb%b3%b4%ea%b3%a0%ec%84%9c","status":"publish","type":"post","link":"https:\/\/blog.agentsoft.co.kr\/index.php\/2026\/07\/29\/47960\/","title":{"rendered":"\uc2dd\ubb3c\ubd84\uc790\uc0dd\ubb3c\ud559\uc2e4\ud5d8 Histochemical GUS assay \uacb0\uacfc\ubcf4\uace0\uc11c"},"content":{"rendered":"

<\/p>\n

\ubaa9\ucc28<\/strong><\/p>\n

1. \uc2e4\ud5d8\uc81c\ubaa9
\n2. \uc2e4\ud5d8\uc77c\uc790
\n3. \uc2e4\ud5d8\uac1c\uc694
\n4. \uc2e4\ud5d8\uc774\ub860
\n5. Materials and methods
\n6. \uc2e4\ud5d8\uacb0\uacfc
\n7. Discussion
\n8. References<\/p>\n

\ubcf8\ubb38\ub0b4\uc6a9<\/strong><\/p>\n

Escherichia coli \u03b2-glucuronidase gene uidA (GUS)\ub97c \uc2dd\ubb3c\uc5d0\uc11c reporter gene\uc73c\ub85c \ud65c\uc6a9\ud558\ub294 \uc5f0\uad6c\ubc29\uc2dd\uc740 1987\ub144 Jefferson \ub4f1\uc5d0 \uc758\ud574 \ub3c4\uc785\ub418\uc5c8\uc73c\uba70(1) \ud604\uc7ac\ub3c4 \ub192\uc740 \uac10\ub3c4, \ud6a8\uc18c \uc548\uc815\uc131 \ubc0f \ud615\uad11 \uce21\uc815, \ubd84\uad11 \uad11\ub3c4 \uce21\uc815 \ub610\ub294 \uc870\uc9c1\ud654\ud559\uc801 \uae30\uc220\uc744 \ud1b5\ud55c \uac80\ucd9c\uc758 \ub2e8\uc21c\uc131\uc73c\ub85c \uc778\ud574 \uc790 \uc8fc \ud65c\uc6a9\ub418\uace0 \uc788\ub2e4. \ud2b9\ud788 GUS\ub294 histochemical staining\uc744 \ud1b5\ud574 endogenous promoter activity\ub97c \uad00\ucc30\ud558\ub294\ub370 \uc788\uc5b4\uc11c \ub9e4\uc6b0 \uc720\uc6a9\ud558\ub2e4.(2)
\n\uc774\ub97c \uc9c4\ud589\ud558\ub294 \uacfc\uc815\uc744 \uc0b4\ud3b4\ubcf4\uba74, transgenic uidA gene\uc744 \uac00\uc9c4 \uc2dd\ubb3c\uc744 5-bromo-4-chloro- 3-indolyl \u00df-d-glucuronide (X-Gluc)\ub85c \ucc98\ub9ac\ud55c\ub2e4. X-Gluc\ub294 GUS\uc5d0 \uc758\ud574 \uc0b0\ud654\uc801\uc73c\ub85c \uc774\ub7c9\uccb4 \ud654\ub418\uba70, GUS \uc5fc\uc0c9\uc758 \ud2b9\uc9d5\uc801 \uccad\uc0c9 \uc0c9\uc18c\uc778 5,5\u2032-dibromo-4,4\u2032-dichloro-indigo\ub97c \uc0dd\uc131\ud558\ub294 \ub450 \uac1c\uc758 indoxyl derivative\ub85c \ubd84\ud574\ub41c\ub2e4.<\/p>\n

\ucd9c\ucc98 : \ud574\ud53c\ucea0\ud37c\uc2a4<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

\ubaa9\ucc28 1. \uc2e4\ud5d8\uc81c\ubaa9 2. \uc2e4\ud5d8\uc77c\uc790 3. \uc2e4\ud5d8\uac1c\uc694 4. \uc2e4\ud5d8\uc774\ub860 5. Materials and methods 6. \uc2e4\ud5d8\uacb0\uacfc 7. Discussion 8. References \ubcf8\ubb38\ub0b4\uc6a9 Escherichia coli \u03b2-glucuronidase gene uidA (GUS)\ub97c \uc2dd\ubb3c\uc5d0\uc11c reporter gene\uc73c\ub85c \ud65c\uc6a9\ud558\ub294 \uc5f0\uad6c\ubc29\uc2dd\uc740 1987\ub144 Jefferson \ub4f1\uc5d0 \uc758\ud574 \ub3c4\uc785\ub418\uc5c8\uc73c\uba70(1) \ud604\uc7ac\ub3c4 \ub192\uc740 \uac10\ub3c4, \ud6a8\uc18c \uc548\uc815\uc131 \ubc0f \ud615\uad11 \uce21\uc815, \ubd84\uad11 \uad11\ub3c4 \uce21\uc815 \ub610\ub294 \uc870\uc9c1\ud654\ud559\uc801 \uae30\uc220\uc744 \ud1b5\ud55c \uac80\ucd9c\uc758 \ub2e8\uc21c\uc131\uc73c\ub85c \uc778\ud574 \uc790 […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[18229,18226,58372,25186,58366,58369,58368,58367,58370],"class_list":{"0":"post-47960","1":"post","2":"type-post","3":"status-publish","4":"format-standard","5":"hentry","6":"category-uncategorized","7":"tag-a--","9":"tag-gus-assay","10":"tag-perovskite","11":"tag-58366","12":"tag-58369","13":"tag-58368","14":"tag-58367","15":"tag-58370"},"_links":{"self":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/47960","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/comments?post=47960"}],"version-history":[{"count":0,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/47960\/revisions"}],"wp:attachment":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/media?parent=47960"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/categories?post=47960"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/tags?post=47960"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}