{"id":50600,"date":"2026-08-24T09:02:32","date_gmt":"2026-08-24T00:02:32","guid":{"rendered":"https:\/\/blog.agentsoft.co.kr\/index.php\/2026\/08\/24\/50600\/"},"modified":"2026-08-24T09:02:32","modified_gmt":"2026-08-24T00:02:32","slug":"%ec%9c%a0%ec%a0%84%ed%95%99%ec%8b%a4%ed%97%98-gene-insertion-deletion","status":"publish","type":"post","link":"https:\/\/blog.agentsoft.co.kr\/index.php\/2026\/08\/24\/50600\/","title":{"rendered":"\uc720\uc804\ud559\uc2e4\ud5d8 Gene insertion & deletion"},"content":{"rendered":"

<\/p>\n

\ubaa9\ucc28<\/strong><\/p>\n

1. Subject
\n2. Object
\n3. Introduction
\n4. Materials & Method
\n5. Result
\n6. Discussion
\n7. Reference<\/p>\n

\ubcf8\ubb38\ub0b4\uc6a9<\/strong><\/p>\n

1.\tSubject : Gene insertion & deletion<\/p>\n

2.\tObject : PCR product\ub97c yeast\uc5d0 transformation \uc2dc\ud0a8 \ub4a4 \uc774\ub97c selection\ud558\uc5ec \uc6d0\ud558\ub294 gene\uc744 \uac00\uc9c4 yeast \ud615\uc131<\/p>\n

3.\tIntroduction
\n–\tPCR
\nPolymerase\uc758 \uc5f0\uc1c4 \ubc18\uc751\uc744 \ud1b5\ud574 DNA\ub97c \ube60\ub974\uac8c \uc99d\ud3ed\uc2dc\ud0a4\ub294 \ubc29\ubc95\uc73c\ub85c Denaturation, Annealing, Elongation\uc758 3\ub2e8\uacc4\ub85c \uad6c\uc131\ub41c\ub2e4, \uc774\ub54c Denaturation\uc5d0\uc11c DNA\ub294 95\u2103\uc758 \uace0\uc628\uc5d0 \uc758\ud574 single strand\uac00 \ub418\uba70, \uc774\ud6c4 Annealing\uc5d0\uc11c primer\uac00 \uacb0\ud569\ud55c\ub2e4. Annealing \ub3c4\ub294 Tm(primer\uc758 \uc808\ubc18\uc740 double strand\ub85c. \ub098\uba38\uc9c0 \uc808\ubc18\uc740 single strand\ub85c \uc874\uc7ac\ud558\ub294\uc628\ub3c4\ub85c \uc77c\ubc18\uc801\uc73c\ub85c (G+C)X4\u2103 + (A+T)X2\u2103\ub85c \uacc4\uc0b0\ub41c\ub2e4)\uac12\uc5d0\uc11c 4~5\u2103 \ub0ae\uc740 \uc628\ub3c4\ub97c \uc774\uc6a9\ud55c\ub2e4. Elongation\uc5d0\uc11c\ub294 \uc57d 75\u2103\uc5d0\uc11c DNA polymerase\uc758 \uc791\uc6a9\uc5d0 \uc758\ud574\uc11c DNA\uc758 \uae38\uc774 \uc2e0\uc7a5\uc774 \uc77c\uc5b4\ub09c\ub2e4. PCR\uc758 \uacb0\uacfc\uac12\uc5d0 \uc601\ud5a5\uc744 \ubbf8\uce58\ub294 \uc694\uc778\uc5d0\ub294 \uac01 \ub2e8\uacc4\uc758 \uc628\ub3c4, cycle \ud69f\uc218, buffer solution, primer, DNA polymerase\uc758 \uc885\ub958 \ub4f1\uc774 \uc788\ub2e4,<\/p>\n

\ucd9c\ucc98 : \ud574\ud53c\ucea0\ud37c\uc2a4<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

\ubaa9\ucc28 1. Subject 2. Object 3. Introduction 4. Materials & Method 5. Result 6. Discussion 7. Reference \ubcf8\ubb38\ub0b4\uc6a9 1. Subject : Gene insertion & deletion 2. Object : PCR product\ub97c yeast\uc5d0 transformation \uc2dc\ud0a8 \ub4a4 \uc774\ub97c selection\ud558\uc5ec \uc6d0\ud558\ub294 gene\uc744 \uac00\uc9c4 yeast \ud615\uc131 3. Introduction – PCR Polymerase\uc758 \uc5f0\uc1c4 \ubc18\uc751\uc744 \ud1b5\ud574 DNA\ub97c \ube60\ub974\uac8c \uc99d\ud3ed\uc2dc\ud0a4\ub294 \ubc29\ubc95\uc73c\ub85c Denaturation, Annealing, […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[23352,60183,46795,60402],"class_list":["post-50600","post","type-post","status-publish","format-standard","hentry","category-uncategorized","tag-23352","tag-60183","tag-46795","tag-60402"],"_links":{"self":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/50600","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/comments?post=50600"}],"version-history":[{"count":0,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/50600\/revisions"}],"wp:attachment":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/media?parent=50600"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/categories?post=50600"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/tags?post=50600"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}