{"id":53313,"date":"2026-09-21T09:00:06","date_gmt":"2026-09-21T00:00:06","guid":{"rendered":"https:\/\/blog.agentsoft.co.kr\/index.php\/2026\/09\/21\/53313\/"},"modified":"2026-09-21T09:00:06","modified_gmt":"2026-09-21T00:00:06","slug":"%ed%99%94%ea%b3%b5%ec%83%9d%eb%ac%bc%ea%b3%b5%ed%95%99%ec%8b%a4%ed%97%98-dna-%ec%a0%9c%ed%95%9c%ed%9a%a8%ec%86%8c-%ec%8b%a4%ed%97%98-%ec%98%88%eb%b9%84%eb%a0%88%ed%8f%ac%ed%8a%b8","status":"publish","type":"post","link":"https:\/\/blog.agentsoft.co.kr\/index.php\/2026\/09\/21\/53313\/","title":{"rendered":"[\ud654\uacf5\uc0dd\ubb3c\uacf5\ud559\uc2e4\ud5d8] DNA \uc81c\ud55c\ud6a8\uc18c \uc2e4\ud5d8 \uc608\ube44\ub808\ud3ec\ud2b8"},"content":{"rendered":"

<\/p>\n

\ubaa9\ucc28<\/strong><\/p>\n

1. \uc2e4\ud5d8 \ubaa9\ud45c<\/p>\n

2. \uc2e4\ud5d8 \uc6d0\ub9ac
\n1) \uc0dd\ubb3c\uc815\ubcf4\ud559(Bioinformatics)
\n2) \uc81c\ud55c\ud6a8\uc18c(Restriction enzyme)
\n(1) I\ud615 \uc81c\ud55c\ud6a8\uc18c
\n(2) II\ud615 \uc81c\ud55c\ud6a8\uc18c
\n(3) III\ud615 \uc81c\ud55c\ud6a8\uc18c
\n3) \uc804\uae30\uc601\ub3d9(Electrophoresis)
\n(1) \uc544\uac00\ub85c\uc2a4 \uac94 \uc804\uae30\uc601\ub3d9(Agarose gel electrophoresis)
\n(2) Electrophoresis Buffer
\n(3) DNA staining dye<\/p>\n

3. \uc2dc\uc57d \uc870\uc0ac & \uc2e4\ud5d8 \uae30\uad6c \ubc0f \ubc29\ubc95
\n1) \uc2e4\ud5d8 \uae30\uad6c
\n2) \uc2e4\ud5d8 \uc2dc\uc57d
\n3) \uc2e4\ud5d8 \ubc29\ubc95<\/p>\n

4. \uc2e4\ud5d8 \uc2dc \uc8fc\uc758\uc0ac\ud56d<\/p>\n

5. \ucc38\uace0 \ubb38\ud5cc<\/p>\n

\ubcf8\ubb38\ub0b4\uc6a9<\/strong><\/p>\n

\uc2e4\ud5d8 \ubaa9\ud45c
\nlambda DNA \ub97c \uc784\uc758\uc758 \uc81c\ud55c\ud6a8\uc18c\ub97c \uc0ac\uc6a9\ud574 \uc808\ub2e8\ud558\uace0, \uc774\ub97c \uc804\uae30\uc601\ub3d9\ud558\uc5ec \uc0ac\uc6a9\ub41c \uc81c\ud55c \ud6a8\uc18c\uc758 \uae30\uc791\uacfc DNA\uc758 \ub300\ub7b5\uc801\uc778 \uad6c\uc870\ub97c \uc54c\uc544\ubcf8\ub2e4. <\/p>\n

<\uc911 \ub7b5><\/p>\n

\uc2e4\ud5d8 \ubc29\ubc95
\n(1) 1.5mL micro tube \uc5d0 \uc544\ub798\uc640 \uac19\uc774 sample\uc744 \uc81c\uc870\ud55c\ub2e4.
\n\u2460 Lambda DNA 5\u03bcl + DW 13\u03bcl + 10x Restriction Enzyme buffer 2\u03bcl
\n\u2461 Lambda DNA 5\u03bcl + DW 12\u03bcl + 10x Restriction Enzyme buffer 2\u03bcl + Restriction Enzyme1 1\u03bcl
\n\u2462 Lambda DNA 5\u03bcl + DW 12\u03bcl + 10x Restriction Enzyme buffer 2\u03bcl + Restriction Enzyme2 1\u03bcl
\n\u2463 Lambda DNA 5\u03bcl + DW 12\u03bcl + 10x Restriction Enzyme buffer 2\u03bcl + Restriction Enzyme1 1\u03bcl + Restriction Enzyme2 1\u03bcl
\n(2) Micro tube \ub97c \ubaa8\ub450 37\u2103\uc5d0\uc11c \ubc18\uc751\uc2dc\ud0a8\ub2e4.
\n(3) DW 49.8ml + 50x TAE buffer 0.02ml \ub97c dilution \ud558\uc5ec 1x TAE buffer 50ml \ub97c \uc81c\uc870\ud55c\ub2e4.
\n(4) Agarose 1%(w\/v)\uc774 \ub418\ub3c4\ub85d 0.5g\uc744 \ub123\uc5b4 \uc804\uccb4 \ubd80\ud53c\uac00 50ml\uc778 1x TAE buffer\ub97c \uc81c\uc870\ud55c\ub2e4.
\n(5) \ub69c\uaed1\uc744 \uc0b4\uc9dd \ub2eb\uc740 \ud6c4 agarose\uac00 \uc644\uc804\ud788 \ub179\uc744 \ub54c\uae4c\uc9c0 \uc804\uc790\ub808\uc778\uc9c0\ub85c \uac00\uc5f4\ud55c\ub2e4.
\n(6) \uc0c1\uc628\uc5d0\uc11c \uc2dd\ud78c \ud6c4, 20000x Redsafe\ub97c2.5\u03bcl \ucca8\uac00\ud558\uc5ec 1x\uac00 \ub418\ub3c4\ub85d \uc6a9\uc561\uc744 \uc81c\uc870\ud55c\ub2e4.
\n(7) Comb \uac00 \uaf42\ud600\uc9c4 \ud2c0\uc5d0 agarose\ub97c \ucc9c\ucc9c\ud788 \ubd93\uace0 \uad73\uc744 \ub54c\uae4c\uc9c0 \uae30\ub2e4\ub9b0\ub2e4.
\n(8) \uac01\uac01\uc758 sample\uc5d0 6x loading buffer \ub97c 4\u03bcl \ucca8\uac00\ud558\uc5ec 1x\uac00 \ub418\ub3c4\ub85d \ub9cc\ub4e0\ub2e4.
\n(9) \uc804\uae30\uc601\ub3d9\uae30\uc5d0 gel\uc774 \uc7a0\uae38 \uc815\ub3c4\ub85c 1x TAE buffer\ub97c \ub123\uc5b4\uc900\ub2e4.
\n(10) Agarose gel well \uc5d0 DNA marker 3\u03bcl\uc640 \uac01\uac01\uc758 sample 5\u03bcl\ub97c loading\ud55c\ub2e4.
\n(11) \uc804\uae30\uc601\ub3d9\uae30 \uc2a4\uc704\uce58\ub97c \ub204\ub978 \ud6c4 gel\uc758 3\/4 \uc815\ub3c4\uc5d0 Orange G\uac00 \uc624\uba74 \uba48\ucd94\uace0 gel\uc744 UV-transi lluminator \ub85c \uad00\ucc30\ud55c\ub2e4.
\n(12) \uac01\uac01\uc758 \uacb0\uacfc\ub97c Ape \ud504\ub85c\uadf8\ub7a8\uc744 \ud1b5\ud574 \ubd84\uc11d\ud55c \ud6c4 \uc0ac\uc6a9\ud55c \uc81c\ud55c\ud6a8\uc18c\uc758 \uc885\ub958\uc640 lambda DNA\uc758 \uad6c\uc870\ub97c \ud30c\uc545\ud55c\ub2e4.<\/p>\n

\ucd9c\ucc98 : \ud574\ud53c\ucea0\ud37c\uc2a4<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

\ubaa9\ucc28 1. \uc2e4\ud5d8 \ubaa9\ud45c 2. \uc2e4\ud5d8 \uc6d0\ub9ac 1) \uc0dd\ubb3c\uc815\ubcf4\ud559(Bioinformatics) 2) \uc81c\ud55c\ud6a8\uc18c(Restriction enzyme) (1) I\ud615 \uc81c\ud55c\ud6a8\uc18c (2) II\ud615 \uc81c\ud55c\ud6a8\uc18c (3) III\ud615 \uc81c\ud55c\ud6a8\uc18c 3) \uc804\uae30\uc601\ub3d9(Electrophoresis) (1) \uc544\uac00\ub85c\uc2a4 \uac94 \uc804\uae30\uc601\ub3d9(Agarose gel electrophoresis) (2) Electrophoresis Buffer (3) DNA staining dye 3. \uc2dc\uc57d \uc870\uc0ac & \uc2e4\ud5d8 \uae30\uad6c \ubc0f \ubc29\ubc95 1) \uc2e4\ud5d8 \uae30\uad6c 2) \uc2e4\ud5d8 \uc2dc\uc57d 3) \uc2e4\ud5d8 \ubc29\ubc95 4. \uc2e4\ud5d8 […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[62027,62026,62028,62025],"class_list":{"0":"post-53313","1":"post","2":"type-post","3":"status-publish","4":"format-standard","5":"hentry","6":"category-uncategorized","7":"tag-dna","9":"tag-62028","10":"tag-62025"},"_links":{"self":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/53313","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/comments?post=53313"}],"version-history":[{"count":0,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/53313\/revisions"}],"wp:attachment":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/media?parent=53313"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/categories?post=53313"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/tags?post=53313"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}