{"id":53333,"date":"2026-09-21T09:00:55","date_gmt":"2026-09-21T00:00:55","guid":{"rendered":"https:\/\/blog.agentsoft.co.kr\/index.php\/2026\/09\/21\/53333\/"},"modified":"2026-09-21T09:00:55","modified_gmt":"2026-09-21T00:00:55","slug":"%ed%99%94%ea%b3%b5%ec%83%9d%eb%ac%bc%ea%b3%b5%ed%95%99%eb%8b%a8%ec%9c%84%ec%a1%b0%ec%9e%91%ec%8b%a4%ed%97%982-pcr-%ed%99%9c%ec%9a%a9%ed%95%9c-%ec%9c%a0%ec%a0%84%ec%9e%90-%ec%a6%9d%ed%8f%ad-%ec%8b%a4-2","status":"publish","type":"post","link":"https:\/\/blog.agentsoft.co.kr\/index.php\/2026\/09\/21\/53333\/","title":{"rendered":"[\ud654\uacf5\uc0dd\ubb3c\uacf5\ud559\ub2e8\uc704\uc870\uc791\uc2e4\ud5d82] PCR \ud65c\uc6a9\ud55c \uc720\uc804\uc790 \uc99d\ud3ed \uc2e4\ud5d8 \uc608\ube44\ub808\ud3ec\ud2b8"},"content":{"rendered":"

<\/p>\n

\ubaa9\ucc28<\/strong><\/p>\n

1. \uc2e4\ud5d8 \ubaa9\ud45c<\/p>\n

2. \uc2e4\ud5d8 \uc6d0\ub9ac
\n1) \uc911\ud569\ud6a8\uc18c \uc5f0\uc1c4 \ubc18\uc751(Polymerase Chain Reaction, PCR)
\n(1) PCR \uacfc\uc815
\n(2) \uc57c\uc0dd\ud615(Wild type)\uacfc \ub3cc\uc5f0\ubcc0\uc774\ud615(Mutant type)
\n2) \ubc18\uc751\uc561\uc758 \uc870\uc131
\n3) \uc804\uae30\uc601\ub3d9 (Electrophoresis)<\/p>\n

3. \uc2dc\uc57d \uc870\uc0ac & \uc2e4\ud5d8 \uae30\uad6c \ubc0f \ubc29\ubc95
\n1) \uc2e4\ud5d8 \uc2dc\uc57d \ubc0f \uae30\uad6c
\n2) \uc2e4\ud5d8 \ubc29\ubc95
\n1. DNA extraction
\n2. Polymerase Chain Reaction
\n3. Electrophoresis<\/p>\n

4. \uc2e4\ud5d8 \uc2dc \uc8fc\uc758\uc0ac\ud56d<\/p>\n

5. \ucc38\uace0 \ubb38\ud5cc<\/p>\n

\ubcf8\ubb38\ub0b4\uc6a9<\/strong><\/p>\n

1. DNA extraction
\n(1) 1\/1000\uc73c\ub85c \ud76c\uc11d\ud55c Proteinase\uc744 1\u3396\uc529 E.P tube\uc5d0 \ub123\ub294\ub2e4.
\n(2) \uba74\ubd09\uc744 \uc0ac\uc6a9\ud574 \uad6c\uac15 \ucc9c\uc7a5\uc744 \uae01\uc5b4 \uc138\ud3ec\ub97c \ubaa8\uc73c\uace0, \ud76c\uc11d\ud55c proteinase solution\uc5d0 \ub123\ub294\ub2e4.
\n(3) Solution\uc744 37\u2103 \uc778\ud050\ubca0\uc774\ud130\uc5d0\uc11c 30\ubd84\uac04 incubation \ud55c\ub2e4.
\n(4) Incubation \ud6c4, \ud55c \uc0d8\ud50c \ub2f9 4\uac1c\uc758 1.5\u3396 EP tube\uc5d0 200uL\uc529 \ub098\ub220 \ub2f4\ub294\ub2e4.
\n(5) (4)\uc5d0\uc11c \uc900\ube44\ub41c tube\uc5d0 20uL\uc758 sodium acetate\uc640 500uL 100% Ethanol\uc744 \uac01\uac01 \ucca8\uac00\ud55c\ub2e4.
\n(6) Tube\ub97c 15\ucd08 \uc815\ub3c4 Vortexing\ud55c \ud6c4 -80\u2103 deep freezer\uc5d0\uc11c 15\ubd84\uac04 \ub0c9\uac01\uc2dc\ud0a8\ub2e4.
\n(7) 15000 rpm, 4\u2103\uc5d0\uc11c 15\ubd84\uac04 \uc6d0\uc2ec\ubd84\ub9ac\ud55c\ub2e4.
\n(8) \uc0c1\uce35\uc561\uc744 \uc81c\uac70\ud55c \ub4a4, 1\u3396\uc758 70% Ethanol\uc744 \ub123\ub294\ub2e4.
\n(9) 15000 rpm, 4\u2103\uc5d0\uc11c 2\ubd84\uac04 \uc6d0\uc2ec\ubd84\ub9ac\ud55c\ub2e4.
\n(10) \uc0c1\uce35\uc561\uc744 \uc81c\uac70\ud55c \ub4a4 \uce68\ucd9c\ub41c DNA\ub97c 20\ubd84\uac04 \uc0c1\uc628\uc5d0\uc11c \uac74\uc870\uc2dc\ud0a8\ub2e4.
\n(11) 50uL\uc758 DDW\ub85c tube(1)\uc5d0\ub9cc \ub123\uc5b4 DNA\ub97c \ud480\uc5b4\uc900 \ub4a4, tube(1)\uc758 \uc6a9\uc561\uc744 \ub2e4\uc74c \ud29c\ube0c\ub85c \uc62e\uaca8 \uac00\uba70 \ub098\uba38\uc9c0 tube\uc758 DNA\ub97c \ud480\uc5b4 \ud558\ub098\ub85c \ub9cc\ub4e0 \ub4a4 vortexing\ud574\uc900\ub2e4.
\n2. Polymerase Chain Reaction
\n(1) \ud55c \uc0d8\ud50c \ub2f9 wild, mutant type \ucd1d 2\uac1c\uc758 Reaction Mixture(W, M)\uc744 \ub9cc\ub4e0\ub2e4.
\nPrimer mix W: 10uM DNA Forward primer + 10uM DNA Wild Reverse primer
\nPrimer mix M: 10uM DNA Forward primer + 10uM DNA Mut. Reverse primer
\n(2)Reaction Mixture\uc758 \uad6c\uc131\uc131\ubd84\uc744 \ubaa8\ub450 \ub123\uace0 10uL \uc774\uc0c1 \ud30c\uc774\ud3ab\uc73c\ub85c pipetting\uc73c\ub85c \uc11e\ub294\ub2e4.
\n(3)PCR \uae30\uae30\uc758 \ub69c\uaed1\uc744 \uc5f4\uace0 Reaction Mixture\uac00 \ub4e0 tube\ub4e4\uc744 \ubaa8\ub450 \ub123\uace0 PCR \uc804\uc6d0\uc744 \ucf20 \ub4a4, cycle \uc218(34X) \ubc0f \uac01 \ub2e8\uacc4\uc758 \uc628\ub3c4\uc640 \uc2dc\uac04\uc744 \uc544\ub798\ub300\ub85c \uc124\uc815\ud55c \ub4a4 run\uc744 \ub20c\ub7ec \uc2dc\uc791\ud55c\ub2e4.
\n3. Electrophoresis
\n(1) 50x TAE buffer\ub97c 1x TAE buffer 500mL\uac00 \ub418\ub3c4\ub85d \uc99d\ub958\uc218\ub85c dilution\ud55c\ub2e4.
\n(2) (1)\uc5d0\uc11c \ub9cc\ub4e0 1X TAE buffer 500mL \uc911 50mL\ub97c \uc791\uc740 Bottle\uc5d0 \ub2f4\uc544\uc8fc\uace0, \uac70\uae30\uc5d0 Agarose \uc9c8\ub7c9\uc774 1%(w\/v)\uac00 \ub418\ub3c4\ub85d \ub123\uc5b4\uc900\ub2e4.
\n(3) \ub69c\uaed1\uc744 \uc0b4\uc9dd\ub9cc \ub2eb\uc740 \ud6c4 \ucda9\ubd84\ud788 \ub179\uc744 \ub54c\uae4c\uc9c0 \uc804\uc790\ub808\uc778\uc9c0\ub85c \uac00\uc5f4\ud55c\ub2e4.
\n(4) \ub9cc\uc84c\uc744 \ub54c \ub530\ub73b\ud55c \uc815\ub3c4\uac00 \ub418\uba74 Redsafe\ub97c \uc804\uccb4\uc758 1\/20000\uc774 \ub418\ub3c4\ub85d \ub123\ub294\ub2e4.
\n(5) \ub179\uc740 agarose \uc6a9\uc561\uc744 \ud2c0\uc5d0 \ucc9c\ucc9c\ud788 \ubd93\uace0 comb\ub97c \uaf42\uc740 \ud6c4 \uad73\uc744 \ub54c\uae4c\uc9c0 \uae30\ub2e4\ub9b0\ub2e4.
\n(6) \uc824\uc774 \uad73\uc73c\uba74 comb\ub97c \uc870\uc2ec\ud788 \ube80 \ud6c4, \ud2c0\uc5d0\uc11c gel\uc744 \ube7c\ub0b4 \uc804\uae30\uc601\ub3d9\uae30\uc5d0 \ub123\uace0 \uc218\ud3c9\uc744 \ub9de\ucd98 \ub4a4 gel\uc774 \ub2e4 \uc7a0\uae38 \uc815\ub3c4\ub85c 1x TAE buffer\ub97c \ubd80\uc5b4\uc900\ub2e4.
\n(7) \uac01\uac01\uc758 sample\uc5d0 6X loading buffer\uac00 1X\uac00 \ub418\ub3c4\ub85d \ud30c\ub77c\ud544\ub984 \uc704\uc5d0\uc11c pipetting\uc73c\ub85c \uc11e\ub294\ub2e4.
\n(8) Agarose gel\uc758 \ud55c well\uc5d0 DNA marker 5uL\ub97c, \ub098\uba38\uc9c0 well\uc5d0 \ud55c well \ub2f9 (sample+loading buffer) mixture\ub97c 10uL\uc529 loading\ud55c\ub2e4.
\n(9) \uc804\uae30\uc601\ub3d9\uae30 \uc2a4\uc704\uce58\ub97c \ub204\ub978 \ub4a4 20\ubd84 \ub3d9\uc548 \uc804\uae30\uc601\ub3d9\uc744 \uc9c4\ud589\ud55c\ub2e4.
\n(10) Gel\uc744 UV-transilluminator\ub85c \uad00\ucc30\ud55c\ub2e4.<\/p>\n

\ucd9c\ucc98 : \ud574\ud53c\ucea0\ud37c\uc2a4<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

\ubaa9\ucc28 1. \uc2e4\ud5d8 \ubaa9\ud45c 2. \uc2e4\ud5d8 \uc6d0\ub9ac 1) \uc911\ud569\ud6a8\uc18c \uc5f0\uc1c4 \ubc18\uc751(Polymerase Chain Reaction, PCR) (1) PCR \uacfc\uc815 (2) \uc57c\uc0dd\ud615(Wild type)\uacfc \ub3cc\uc5f0\ubcc0\uc774\ud615(Mutant type) 2) \ubc18\uc751\uc561\uc758 \uc870\uc131 3) \uc804\uae30\uc601\ub3d9 (Electrophoresis) 3. \uc2dc\uc57d \uc870\uc0ac & \uc2e4\ud5d8 \uae30\uad6c \ubc0f \ubc29\ubc95 1) \uc2e4\ud5d8 \uc2dc\uc57d \ubc0f \uae30\uad6c 2) \uc2e4\ud5d8 \ubc29\ubc95 1. DNA extraction 2. Polymerase Chain Reaction 3. Electrophoresis 4. \uc2e4\ud5d8 […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[58371,62076,62077,62075],"class_list":{"0":"post-53333","1":"post","2":"type-post","3":"status-publish","4":"format-standard","5":"hentry","6":"category-uncategorized","7":"tag-colony-pcr","8":"tag-pcr","10":"tag-62075"},"_links":{"self":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/53333","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/comments?post=53333"}],"version-history":[{"count":0,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/53333\/revisions"}],"wp:attachment":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/media?parent=53333"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/categories?post=53333"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/tags?post=53333"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}