{"id":7048,"date":"2025-06-15T09:00:20","date_gmt":"2025-06-15T00:00:20","guid":{"rendered":"https:\/\/blog.agentsoft.co.kr\/index.php\/2025\/06\/15\/7048\/"},"modified":"2025-06-15T09:00:20","modified_gmt":"2025-06-15T00:00:20","slug":"%eb%af%b8%ec%83%9d%eb%ac%bc%ed%95%99-transformation-%ed%98%95%ec%a7%88%ec%a0%84%ed%99%98-%ec%8b%a4%ed%97%98-%eb%a0%88%ed%8f%ac%ed%8a%b8","status":"publish","type":"post","link":"https:\/\/blog.agentsoft.co.kr\/index.php\/2025\/06\/15\/7048\/","title":{"rendered":"\ubbf8\uc0dd\ubb3c\ud559 transformation \ud615\uc9c8\uc804\ud658 \uc2e4\ud5d8 \ub808\ud3ec\ud2b8"},"content":{"rendered":"

<\/p>\n

\ubaa9\ucc28<\/strong><\/p>\n

1.\uc2e4\ud5d8\uc81c\ubaa9(title)
\n 2.\uc2e4\ud5d8\ub0a0\uc9dc(date):
\n 3.\uc2e4\ud5d8\ubaa9\uc801(purpose):
\n4.\uc2e4\ud5d8 \uc900\ube44\ubb3c(material-reagent, instrument)
\n 5.\uc2e4\ud5d8 \ubc29\ubc95(method)
\n6.\uc2e4\ud5d8 \uacb0\uacfc(result)
\n7.\uc2e4\ud5d8 \uace0\ucc30(discussion): \uc2e4\ud5d8\uc5d0 \ub300\ud55c \uace0\ucc30\ub3c4 \ucd94\uac00\ud558\uae30
\n8.\ucc38\uace0\ubb38\ud5cc(reference)<\/p>\n

\ubcf8\ubb38\ub0b4\uc6a9<\/strong><\/p>\n

\uc2e4\ud5d8\uc81c\ubaa9(title): Transformation
\n\u00b7 \uc2e4\ud5d8\ub0a0\uc9dc(date):
\n\u00b7 \uc2e4\ud5d8\ubaa9\uc801(purpose): plasmid DNA\ub97c competent cell\uc5d0 \ub3c4\uc785\ud558\uc5ec colony\ub97c \uad00\ucc30\ud568\uc73c\ub85c\uc368 transformation\uacfc\uc815\uc744 \uc774\ud574\ud55c\ub2e4.
\n\u00b7 \uc2e4\ud5d8 \uc900\ube44\ubb3c(material-reagent, instrument)
\n\ud53c\ud3ab, LB agar \ubc30\uc9c0, spreader, DH5 alpha, \ud56d\uc628\uc218\uc870, \uc5bc\uc74c\uc218\uc870, e-tube, dilution\ud55c DNA<\/p>\n

\u00b7 \uc2e4\ud5d8 \ubc29\ubc95(method)
\n\u2460 DH5 alpha\ub97c \uc5bc\uc74c\uc774 \ub2f4\uae34 \uc218\uc870\uc5d0 \ub450\uace0, e-tube\ub97c \uc5bc\uc74c\uc5d0 \ub450\uc5b4 \ucc28\uac11\uac8c \uc720\uc9c0\ud55c\ub2e4.
\n\u2461 20\u03bcl\uc758 DH5 alpha\ub97c e-tube\uc5d0 \ub123\ub294\ub2e4.
\n\u2462 DNA 2\u03bcl\uc744 DH5 alpha\uc5d0 \ub123\ub294\ub2e4. (\ucd5c\ub300\ud55c competent cell\uc774 \ucda9\uaca9\uc744 \ubc1b\uc9c0 \uc54a\ub3c4\ub85d \uc8fc\uc758\ud55c\ub2e4.)
\n\u2463 \uc5bc\uc74c\uc5d0\uc11c 30\ubd84\uac04 incubation\ud55c\ub2e4.
\n\u2464 42\u2103\uc5d0\uc11c 90sec\uac04 incubation\ud55c\ub2e4.
\n\u2465 \uc5bc\uc74c\uc5d0\uc11c 5\ubd84\uac04 incubation\ud55c\ub2e4.
\n\u2466 37\u2103\uc5d0\uc11c \ub370\uc6b4 \ud56d\uc0dd\uc81c\uac00 \ub4e4\uc5b4\uc788\ub294 LB agar \ubc30\uc9c0\uc5d0 competent cell 20\u03bcl\uc744 \ub123\uace0 spreading\ud55c\ub2e4.
\n (control group)
\n\u2467 37\u2103\uc5d0\uc11c \ub370\uc6b4 \ud56d\uc0dd\uc81c\uac00 \ub4e4\uc5b4\uc788\ub294 LB agar \ubc30\uc9c0\uc5d0 transformation\ud55c dna\ub97c 20\u03bcl \ub123\uace0 spreading\ud55c\ub2e4.
\n\u2468 37\u2103\uc5d0\uc11c overnight\uc73c\ub85c \ubc30\uc591\ud55c\ub2e4.<\/p>\n

\u00b7 \uc2e4\ud5d8 \uacb0\uacfc(result)<\/p>\n

\u00b7 \uc2e4\ud5d8 \uace0\ucc30(discussion): \uc2e4\ud5d8\uc5d0 \ub300\ud55c \uace0\ucc30\ub3c4 \ucd94\uac00\ud558\uae30
\ncompetent cell\uc740 \uc628\ub3c4\uc640 \ucda9\uaca9\uc5d0 \ubbfc\uac10\ud558\ubbc0\ub85c \uc2e4\ud5d8 \uc2dc \uc8fc\uc758\ud574\uc11c \ub2e4\ub904\uc57c\ud55c\ub2e4. \ub610\ud55c \ubc30\uc9c0\uc5d0 spreading\uc2dc \ubc30\uc9c0\uac00 \ud30c\uc774\uc9c0 \uc54a\ub3c4\ub85d \uc870\uc2ec\ud574\uc11c \ub2e4\ub904\uc57c \ud55c\ub2e4.<\/p>\n

\ucd9c\ucc98 : \ud574\ud53c\ucea0\ud37c\uc2a4<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

\ubaa9\ucc28 1.\uc2e4\ud5d8\uc81c\ubaa9(title) 2.\uc2e4\ud5d8\ub0a0\uc9dc(date): 3.\uc2e4\ud5d8\ubaa9\uc801(purpose): 4.\uc2e4\ud5d8 \uc900\ube44\ubb3c(material-reagent, instrument) 5.\uc2e4\ud5d8 \ubc29\ubc95(method) 6.\uc2e4\ud5d8 \uacb0\uacfc(result) 7.\uc2e4\ud5d8 \uace0\ucc30(discussion): \uc2e4\ud5d8\uc5d0 \ub300\ud55c \uace0\ucc30\ub3c4 \ucd94\uac00\ud558\uae30 8.\ucc38\uace0\ubb38\ud5cc(reference) \ubcf8\ubb38\ub0b4\uc6a9 \uc2e4\ud5d8\uc81c\ubaa9(title): Transformation \u00b7 \uc2e4\ud5d8\ub0a0\uc9dc(date): \u00b7 \uc2e4\ud5d8\ubaa9\uc801(purpose): plasmid DNA\ub97c competent cell\uc5d0 \ub3c4\uc785\ud558\uc5ec colony\ub97c \uad00\ucc30\ud568\uc73c\ub85c\uc368 transformation\uacfc\uc815\uc744 \uc774\ud574\ud55c\ub2e4. \u00b7 \uc2e4\ud5d8 \uc900\ube44\ubb3c(material-reagent, instrument) \ud53c\ud3ab, LB agar \ubc30\uc9c0, spreader, DH5 alpha, \ud56d\uc628\uc218\uc870, \uc5bc\uc74c\uc218\uc870, e-tube, dilution\ud55c DNA \u00b7 \uc2e4\ud5d8 \ubc29\ubc95(method) \u2460 DH5 […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[12090,12088,12087,9093],"class_list":["post-7048","post","type-post","status-publish","format-standard","hentry","category-uncategorized","tag-transformation","tag-12088","tag-12087","tag-9093"],"_links":{"self":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/7048","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/comments?post=7048"}],"version-history":[{"count":0,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/7048\/revisions"}],"wp:attachment":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/media?parent=7048"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/categories?post=7048"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/tags?post=7048"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}