{"id":7108,"date":"2025-06-15T09:01:07","date_gmt":"2025-06-15T00:01:07","guid":{"rendered":"https:\/\/blog.agentsoft.co.kr\/index.php\/2025\/06\/15\/7108\/"},"modified":"2025-06-15T09:01:07","modified_gmt":"2025-06-15T00:01:07","slug":"%ec%84%9c%ea%b0%95%eb%8c%80-%ea%b8%b0%ea%b8%b0%eb%b6%84%ec%84%9d%ec%8b%a4%ed%97%98-hplc-%eb%a0%88%ed%8f%ac%ed%8a%b8","status":"publish","type":"post","link":"https:\/\/blog.agentsoft.co.kr\/index.php\/2025\/06\/15\/7108\/","title":{"rendered":"\uc11c\uac15\ub300 \uae30\uae30\ubd84\uc11d\uc2e4\ud5d8 < HPLC > \ub808\ud3ec\ud2b8"},"content":{"rendered":"

<\/p>\n

\ubaa9\ucc28<\/strong><\/p>\n

1. ABSTRACT<\/p>\n

2. INTRODUCTION
\n 1) \uc2e4\ud5d8\ubaa9\uc801
\n 2) Theories<\/p>\n

3. EXPERIMENTAL
\n 1) \uc0ac\uc6a9\ud55c \uc2dc\uc57d \ubc0f \uae30\uad6c
\n 2) \uc2e4\ud5d8 \ubc29\ubc95<\/p>\n

4. RESULTS AND DISCUSSION<\/p>\n

5. CONCLUSION<\/p>\n

6. REFERENCES<\/p>\n

\ubcf8\ubb38\ub0b4\uc6a9<\/strong><\/p>\n

ABSTRACT.
\nHPLC\uc640 C18 \uceec\ub7fc\uc744 \uc774\uc6a9\ud558\ub294 \uc5ed\uc0c1\ud06c\ub85c\ub9c8\ud1a0 \uadf8\ub798\ud53c\ub97c \uc9c4\ud589\ud558\uc5ec Uric acid\uc758 \ub18d\ub3c4 \ubcc4 \ud06c\ub85c\ub9c8\ud1a0\uadf8\ub7a8\uc744 \uc5bb\uc5c8\ub2e4. \uc774\ub97c \ubd84\uc11d\ud558\uc5ec \ub18d\ub3c4\uac00 \uc99d\uac00\ud568\uc5d0 \ub530\ub77c peak Hegiht\uac00 \uc99d\uac00\ud558\ub294 \uac83\uc744 \uc54c \uc218 \uc788\uc5c8\ub2e4. Uric aicd\uc640 Uracil\uc758 peak\uac00 \ubb34\uc5c7\uc778\uc9c0 \ubd84\uc11d\ud574\ubcf4\uc558\uace0, \uadf8 \uacb0\uacfc, retention time\uc774 \uc9e7\uc544 \ud06c\ub85c\ub9c8\ud1a0\uadf8\ub7a8\uc5d0\uc11c \uc67c\ucabd\uc5d0 \uc704\uce58\ud55c peak\uc774 Uracil\uc774 \uac83\uc784\uc744 \ubc1d\ud614\ub2e4. peak Area \uc815\ubcf4\ub97c \ud65c\uc6a9\ud574 Calibration \uace1\uc120\uc744 \uadf8\ub838\ub2e4. 1\ucc28 \ud568\uc218\uc2dd\uc744 \uc5bb\uc5b4 unknown sample\uc758 \ub18d\ub3c4\ub97c \uacc4\uc0b0\ud588\uace0, \uc0d8\ud50c \ub0b4 Uric acid\uc758 \ub18d\ub3c4\uac00 24.665ppm\uc774\ub77c\ub294 \uac83\uc744 \ubc1d\ud614\ub2e4. reference data\uc5d0\uc11c \uc774\ub860\ub2e8 \uc218\uc640 \uc774\ub860\ub2e8 \ub192\uc774\ub97c \uacc4\uc0b0\ud558\uace0, \uc774\ub4e4\uacfc \ubd84\ub9ac\ub2a5\uc758 \uad00\uacc4\ub3c4 \uc54c\uc544\ubcf4\uc558\ub2e4.<\/p>\n

Key words: High performance liquid chromatography, retention time, standard curve, \uc774\ub860\ub2e8 \uc218, \uc774\ub860\ub2e8 \ub192\uc774<\/p>\n

INTRODUCTION
\n1. \uc2e4\ud5d8 \ubaa9\uc801
\nHPLC \uc758 \uc0ac\uc6a9 \ubc29\ubc95\uc744 \uc775\ud600 standard curve \ub97c \uc774\uc6a9\ud558\uc5ec \ubbf8\uc9c0 \uc2dc\ub8cc\uc758 \ub18d\ub3c4\ub97c \ud655\uc778\ud55c\ub2e4. Peak width \uc640 retention time \uc744 \ud1b5\ud558\uc5ec \uc774\ub860 \ub2e8\uc218\uc640 \uc774\ub860 \ub2e8\ub192\uc774\uc758 \uac1c\ub150\uc744 \uc774\ud574\ud55c\ub2e4.<\/p>\n

2. Theories
\n2.1 HPLC
\n\ud06c\ub85c\ub9c8\ud1a0\uadf8\ub798\ud53c\ub294 \uc815\uc9c0\uc0c1\uc5d0 \ub300\ud55c \uc774\ub3d9\uc0c1\uc758 \uc18d\ub3c4 \ucc28\uc774\ub85c \ubb3c\uc9c8\uc744 \ubd84\ub9ac\ud558\ub294 \uae30\uc220\uc774\ub2e4. \uc774\ub294 GC, LC, HPLC, UHPLC \ub4f1\uc73c\ub85c \ub098\ub25c\ub2e4. LC \ub294 \uc774\ub3d9\uc0c1\uc774 \uc561\uccb4\uc778 \uc561\uccb4 \ud06c\ub85c\ub9c8\ud1a0\uadf8\ub798\ud53c\uc774\ub2e4. \uceec\ub7fc\uc5d0 \uc815\uc9c0\uc0c1\uc744 \ucc44\uc6b4 \ud6c4 \uc774\ub3d9\uc0c1\uc744 \ud758\ub824\uc900\ub2e4. \uc815\uc9c0\uc0c1\uacfc \uc0c1\ud638\uc791\uc6a9\uc774 \ub9ce\uc744\uc218\ub85d \uceec\ub7fc \ub0b4\uc5d0 \uc624\ub798 \uba38\ubb3c\ub7ec \uc788\ub294\ub2e4. \uc774\ub54c \uc555\ub825\uc744 \uac00\ud558\uba74 \ubb3c\uc9c8\uc774 \ub354 \ube68\ub9ac \uceec\ub7fc\uc5d0\uc11c \ub0b4\ub824\uc628\ub2e4. \uc774\uc5d0 6000 psi \uae4c\uc9c0 \uace0\uc555\ub825\uc744 \uac78\uc5b4\uc8fc\ub294 \uac83\uc774 HPLC(High Performance Liquid Chromatography) \uc774\ub2e4. UHPLC \ub294 15000psi \uae4c\uc9c0 \uc555\ub825\uc744 \uac00\ud560 \uc218 \uc788\ub2e4.<\/p>\n

\ucd9c\ucc98 : \ud574\ud53c\ucea0\ud37c\uc2a4<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

\ubaa9\ucc28 1. ABSTRACT 2. INTRODUCTION 1) \uc2e4\ud5d8\ubaa9\uc801 2) Theories 3. EXPERIMENTAL 1) \uc0ac\uc6a9\ud55c \uc2dc\uc57d \ubc0f \uae30\uad6c 2) \uc2e4\ud5d8 \ubc29\ubc95 4. RESULTS AND DISCUSSION 5. CONCLUSION 6. REFERENCES \ubcf8\ubb38\ub0b4\uc6a9 ABSTRACT. HPLC\uc640 C18 \uceec\ub7fc\uc744 \uc774\uc6a9\ud558\ub294 \uc5ed\uc0c1\ud06c\ub85c\ub9c8\ud1a0 \uadf8\ub798\ud53c\ub97c \uc9c4\ud589\ud558\uc5ec Uric acid\uc758 \ub18d\ub3c4 \ubcc4 \ud06c\ub85c\ub9c8\ud1a0\uadf8\ub7a8\uc744 \uc5bb\uc5c8\ub2e4. \uc774\ub97c \ubd84\uc11d\ud558\uc5ec \ub18d\ub3c4\uac00 \uc99d\uac00\ud568\uc5d0 \ub530\ub77c peak Hegiht\uac00 \uc99d\uac00\ud558\ub294 \uac83\uc744 \uc54c \uc218 \uc788\uc5c8\ub2e4. Uric […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[4587,12202,12203,12179,12181,12182,5955,12205,12204,12180],"class_list":["post-7108","post","type-post","status-publish","format-standard","hentry","category-uncategorized","tag-hplc","tag-retention-time","tag-standard-curve","tag-12179","tag-12181","tag-12182","tag-5955","tag-12205","tag-12204","tag-12180"],"_links":{"self":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/7108","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/comments?post=7108"}],"version-history":[{"count":0,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/7108\/revisions"}],"wp:attachment":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/media?parent=7108"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/categories?post=7108"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/tags?post=7108"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}