{"id":8763,"date":"2025-07-02T09:00:39","date_gmt":"2025-07-02T00:00:39","guid":{"rendered":"https:\/\/blog.agentsoft.co.kr\/index.php\/2025\/07\/02\/8763\/"},"modified":"2025-07-02T09:00:39","modified_gmt":"2025-07-02T00:00:39","slug":"%ec%a3%bc%ec%a0%9c%ed%83%90%ea%b5%ac%eb%b3%b4%ea%b3%a0%ec%84%9c-%ed%94%84%eb%9d%bc%ec%9e%84-%ec%97%90%eb%94%94%ed%84%b0","status":"publish","type":"post","link":"https:\/\/blog.agentsoft.co.kr\/index.php\/2025\/07\/02\/8763\/","title":{"rendered":"\uc8fc\uc81c\ud0d0\uad6c\ubcf4\uace0\uc11c – \ud504\ub77c\uc784 \uc5d0\ub514\ud130"},"content":{"rendered":"

<\/p>\n

\ubaa9\ucc28<\/strong><\/p>\n

\u2160. \uc11c\ub860
\n 1. \uc5f0\uad6c \ubc30\uacbd3
\n 2. \uc774\ub860\uc801 \ubc30\uacbd3
\n 3. \ud604\uc7ac \uc0c1\ud6694<\/p>\n

\u2161. \ubcf8\ub860
\n 1. \uae30\uc220\uc801 \uce21\uba745
\n 2. \uc0dd\uba85\uacfc\ud559\uc801 \uce21\uba746
\n 3. \uc724\ub9ac\uc801 \uce21\uba747<\/p>\n

\u2162. \uacb0\ub860 \ubc0f \uc81c\uc5b88<\/p>\n

\u2163. \ucc38\uace0 \ubb38\ud5cc9<\/p>\n

\ubcf8\ubb38\ub0b4\uc6a9<\/strong><\/p>\n

1. \uc5f0\uad6c \ubc30\uacbd<\/p>\n

\ucef4\ud4e8\ud130 \uc6cc\ub4dc\ud504\ub85c\uc138\uc11c\ub85c \uae00\uc790\ub97c \uc4f0\uace0 \uc9c0\uc6b0\ub294 \uac83\ucc98\ub7fc \uc778\uccb4 DNA \uc720\uc804\ucf54\ub4dc\ub97c \ud558\ub098\ud558\ub098 \uc790\uc720\uc790\uc7ac\ub85c \ud3b8\uc9d1\ud560 \uc218 \uc788\ub294 \ucc28\uc138\ub300 \uc720\uc804\uc790 \uad50\uc815 \uae30\uc220\uc774 \ud0c4\uc0dd\ud588\ub2e4. \uc774 \uae30\uc220\uc744 \uc801\uc6a9\ud558\uba74 \ud604\uc874\ud558\ub294 \uc720\uc804\uc9c8\ud658 \uc911 90%\ub97c \uce58\ub8cc\ud560 \uc218 \uc788\uc744 \uac83\uc73c\ub85c \uae30\ub300\ub41c\ub2e4. \uc2e0\uc885 \ucf54\ub85c\ub098 \ubc14\uc774\ub7ec\uc2a4\ub85c \ubab8\uc0b4\uc744 \uc553\uace0 \uc788\ub294 \uc804\uc138\uacc4 \uc778\ub958\uc5d0\uac8c\ub294 \uae30\uc874 3\uc138\ub300 \ud06c\ub9ac\uc2a4\ud37c \uac00\uc704\uac00 \uac00\uc9c0\uace0 \uc788\ub294 \ub2e8\uc810\uc744 \ubcf4\uc644\ud560 \uc218 \uc788\ub294 \ud504\ub77c\uc784 \uc5d0\ub514\ud130\uc758 \ucd9c\ud604\uc774 \ubc18\uac00\uc6b4 \uc18c\uc2dd\uc774 \uc544\ub2d0 \uc218 \uc5c6\ub2e4.
\n\ud558\uc9c0\ub9cc \uae30\uc874 3\uc138\ub300 \ud06c\ub9ac\uc2a4\ud37c \uac00\uc704\uc640 \ubb34\uc5c7\uc774, \uc5b4\ub5a0\ud55c \uc810\uc774 \ub2e4\ub978\uc9c0\ub97c \ubd84\uc11d\ud558\uace0 \uc5b4\ub5a0\ud55c \uc810\uc744 \uc751\uc6a9\ud574 \ud604\ub300 \uc758\ud559\ubd80\ubb38 \ud6a8\uacfc\ub97c \ub3c4\ucd9c\ud574\ub0bc \uc218 \uc788\ub294\uc9c0 \uc758\ubb38\uc774\uc5c8\uace0, \uadf8\ub9ac\ud558\uc5ec \ud504\ub77c\uc784 \uc5d0\ub514\ud130\ub97c \uc0dd\uba85\uacfc\ud559\uc801, \uae30\uc220\uc801, \uc724\ub9ac\uc801 \uce21\uba74\uc5d0\uc11c \uc138\ubc00\ud558\uac8c \ub2e4\ub904\ubcf4\uace0\uc790 \ud558\uc5ec \ubcf8 \uc5f0\uad6c\ub97c \uc9c4\ud589\ud558\uac8c \ub418\uc5c8\ub2e4.<\/p>\n

2. \uc774\ub860\uc801 \ubc30\uacbd<\/p>\n

\uac00. \uc720\uc804\uc790 \uac00\uc704\ub780 DNA nuclease \uae30\ub2a5\uc744 \uac16\ub294 \ub2e8\ubc31\uc9c8 \ubcf5\ud569\uccb4\ub97c \uc774\uc6a9\ud558\uc5ec \uc720\uc804\uccb4 \ud2b9\uc815 \ubd80\uc704\uc5d0 DNA\uc758 \uc0bd\uc785, \uacb0\uc190 \ubc0f \uce58\ud658\uc744 \uc720\ub3c4\ud558\uc5ec \ub3cc\uc5f0\ubcc0\uc774\ub97c \uc77c\uc73c\ud0a4\ub294 \uae30\uc220\uc744 \ub9d0\ud55c\ub2e4.\u00b9\u207e
\n\ub098. \ud06c\ub9ac\uc2a4\ud37c\ub294 DNA\uc0c1\uc5d0 \uc874\uc7ac\ud558\ub294 18~40\uac1c\ub85c \uad6c\uc131\ub41c \ud2b9\uc815 \uc5fc\uae30\uc11c\uc5f4\uc744 \uc778\uc2dd\ud558\uc5ec DNA \ub450 \uac00\ub2e5\uc744 \uc808\ub2e8\ud558\ub294 \uc778\uacf5 \uc81c\ud55c\ud6a8\uc18c\ub85c \ub3d9\uc2dd\ubb3c \uc720\uc804\uc790\uc758 \uc190\uc0c1\ub41c DNA\ub97c \uc798\ub77c\ub0b4\uace0 \uc815\uc0c1 DNA\ub85c \uad50\uccb4\ud558\ub294 \uc720\uc804\uc790 \ud3b8\uc9d1 \uae30\uc220\uc744 \uc758\ubbf8\ud55c\ub2e4.\u00b2\u207e
\n\ub2e4. \ud06c\ub9ac\uc2a4\ud37c \uce74\uc2a49\ub294 \ud06c\ub9ac\uc2a4\ud37c\uc640 Cas9 \ub2e8\ubc31\uc9c8 \ud6a8\uc18c\ub97c \ud568\uaed8 \uc0ac\uc6a9\ud558\ub294 \uc720\uc804\uc790 \ud3b8\uc9d1 \uae30\uc220\uc774\ub2e4.\u00b3\u207e
\n\ub77c. \uc5ed\uc804\uc0ac\ud6a8\uc18c\ub294 RNA\ub97c \uc8fc\ud615\ud2c0\ub85c \uc0bc\uc544 RNA \uc11c\uc5f4\uc5d0 \uc0c1\ubcf4\uc801\uc778 DNA\ub97c \ud569\uc131\ud574\ub0b4\ub294 \ub2a5\ub825\uc744 \uac16\uace0 \uc788\ub294 \ud6a8\uc18c\uc774\ub2e4.\u2074\u207e
\n\ub9c8. \ud504\ub77c\uc784 \uc5d0\ub514\ud130\ub294 \ud06c\ub9ac\uc2a4\ud37c \uce74\uc2a49\ub97c \ub9e4\uac1c\ub85c \ud558\ub294 \uc720\uc804\uccb4 \uad50\uc815 \uae30\uc220 \ub610\ub294 \ub3c4\uad6c\uc758 \ud558\ub098\ub85c\uc11c, \ud45c\uc801 DNA\uc5d0 \ub2e4\uc591\ud55c \ud06c\uae30\uc758 DNA \ubd80\uc704\uc758 \uc0bd\uc785 \ub610\ub294 \uacb0\uc2e4 \ubcc0\uc774 \uadf8\ub9ac\uace0 DNA\uc758 \ub124 \uac00\uc9c0 \uc5fc\uae30\uc11c\uc5f4\uc744 \uc6d0\ud558\ub294 \ub2e4\ub978 \uc5fc\uae30\uc11c\uc5f4\ub85c \uce58\ud658\uc2dc\ud0ac \uc218 \uc788\ub3c4\ub85d \uac1c\ubc1c\ub41c \uae30\uc220 \ub610\ub294 \ub3c4\uad6c. \ud06c\ub9ac\uc2a4\ud37c \uc720\uc804\uc790 \uac00\uc704 \ub3c4\uad6c\uc778 \ud06c\ub9ac\uc2a4\ud37c \uce74\uc2a49\uc758 2\uc911 \uac00\ub2e5 \uc808\ub2e8\uc774 \uc544\ub2cc \ud55c \uac00\ub2e5\uc5d0 \ud2c8\uc744 \ub9cc\ub4e4\uc5b4\ub0b4\ub294 \ub2c8\ucf00\uc774\uc988(nickase) \uae30\ub2a5\uc758 \ubcc0\ud615\ub41c \uce74\uc2a49 \ub2e8\ubc31\uc9c8\uc5d0 RNA\uc5d0\uc11c DNA\ub97c \ubcf5\uc81c\ud558\ub294 \uae30\ub2a5\uc744 \uac00\uc9c4 \uc5ed\uc804\uc0ac\ud6a8\uc18c\ub97c \uc735\ud569\ud558\uace0 \uae30\ubcf8 \ud06c\ub9ac\uc2a4\ud37c \uc720\uc804\uc790 \uac00\uc704 \ub3c4\uad6c\uc5d0 \uc0ac\uc6a9\ub418\ub294 \ub2e8\uc77c \uac00\ub2e5 \uac00\uc774\ub4dc RNA\uc758 3′ \ub9d0\ub2e8 \ubd80\uc704\uc5d0 \ud45c\uc801 DNA\uc758 \uc77c\ubd80\uc640 \uacb0\ud569\ub418\ub3c4\ub85d \uc124\uacc4\ub41c \uac00\ub2e5\uacfc \ubcc0\uc774\ub97c \ub3c4\uc785\uc2dc\ud0a4\ub294 \ubcc0\uc774\uac00\ub2e5\uc744 \ud568\uaed8 \uc5f0\uacb0\ud55c \ud504\ub77c\uc784 \uc5d0\ub514\ud130 \uac00\uc774\ub4dc RNA(pegRNA)\ub97c \uc0ac\uc6a9\ud55c\ub2e4.<\/p>\n

\ucd9c\ucc98 : \ud574\ud53c\ucea0\ud37c\uc2a4<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

\ubaa9\ucc28 \u2160. \uc11c\ub860 1. \uc5f0\uad6c \ubc30\uacbd3 2. \uc774\ub860\uc801 \ubc30\uacbd3 3. \ud604\uc7ac \uc0c1\ud6694 \u2161. \ubcf8\ub860 1. \uae30\uc220\uc801 \uce21\uba745 2. \uc0dd\uba85\uacfc\ud559\uc801 \uce21\uba746 3. \uc724\ub9ac\uc801 \uce21\uba747 \u2162. \uacb0\ub860 \ubc0f \uc81c\uc5b88 \u2163. \ucc38\uace0 \ubb38\ud5cc9 \ubcf8\ubb38\ub0b4\uc6a9 1. \uc5f0\uad6c \ubc30\uacbd \ucef4\ud4e8\ud130 \uc6cc\ub4dc\ud504\ub85c\uc138\uc11c\ub85c \uae00\uc790\ub97c \uc4f0\uace0 \uc9c0\uc6b0\ub294 \uac83\ucc98\ub7fc \uc778\uccb4 DNA \uc720\uc804\ucf54\ub4dc\ub97c \ud558\ub098\ud558\ub098 \uc790\uc720\uc790\uc7ac\ub85c \ud3b8\uc9d1\ud560 \uc218 \uc788\ub294 \ucc28\uc138\ub300 \uc720\uc804\uc790 \uad50\uc815 \uae30\uc220\uc774 \ud0c4\uc0dd\ud588\ub2e4. \uc774 \uae30\uc220\uc744 […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[9088,2354,2636,3664,2920,14018,13992,14019,13975],"class_list":["post-8763","post","type-post","status-publish","format-standard","hentry","category-uncategorized","tag-dna","tag-2--","tag-2636","tag-3664","tag-2920","tag-14018","tag-13992","tag-14019","tag-13975"],"_links":{"self":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/8763","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/comments?post=8763"}],"version-history":[{"count":0,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/posts\/8763\/revisions"}],"wp:attachment":[{"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/media?parent=8763"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/categories?post=8763"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/blog.agentsoft.co.kr\/index.php\/wp-json\/wp\/v2\/tags?post=8763"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}